Genomic description of critical cannabinoid biosynthesis genes

IF 1 4区 生物学 Q3 PLANT SCIENCES Botany Pub Date : 2023-04-14 DOI:10.1139/cjb-2022-0140
Peter A. Innes, D. Vergara
{"title":"Genomic description of critical cannabinoid biosynthesis genes","authors":"Peter A. Innes, D. Vergara","doi":"10.1139/cjb-2022-0140","DOIUrl":null,"url":null,"abstract":"Cannabinoid production is a key attribute of the plant Cannabis sativa and characterizing the genes involved is an essential first step to develop tools for their optimization. We used bioinformatic approaches to annotate and explore variation in the genes coding for enzymes comprising the cannabinoid pathway: olivetol synthase (OLS), olivetolic acid cyclase (OAC), cannabigerolic acid synthase (CBGAS), and the cannabinoid oxidocyclases (THCAS, CBDAS, CBCAS) in multiple C. sativa genome assemblies from diverse lineages. The former three enzymes generate the precursor molecules for the oxidocyclases to produce cannabinoids THC and CBD. We show that duplications of OLS and OAC are consistent across varieties, and that OAC has the least amount of sequence diversity based on phylogenetic comparisons. We also found that one CBGAS-like gene exhibits copy number variation among varieties. We discuss implications of these genes existing on separate chromosomes (with homologs of each found in close proximity), and the significance of CBGAS being located on the X chromosome for cannabinoid production in female plants. This study provides valuable insight on the genomic identity and variation of cannabinoid biosynthesis genes that will benefit future research on the origin and evolution of this pathway, a driver of economic, social, and medicinal value.","PeriodicalId":9092,"journal":{"name":"Botany","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1139/cjb-2022-0140","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Cannabinoid production is a key attribute of the plant Cannabis sativa and characterizing the genes involved is an essential first step to develop tools for their optimization. We used bioinformatic approaches to annotate and explore variation in the genes coding for enzymes comprising the cannabinoid pathway: olivetol synthase (OLS), olivetolic acid cyclase (OAC), cannabigerolic acid synthase (CBGAS), and the cannabinoid oxidocyclases (THCAS, CBDAS, CBCAS) in multiple C. sativa genome assemblies from diverse lineages. The former three enzymes generate the precursor molecules for the oxidocyclases to produce cannabinoids THC and CBD. We show that duplications of OLS and OAC are consistent across varieties, and that OAC has the least amount of sequence diversity based on phylogenetic comparisons. We also found that one CBGAS-like gene exhibits copy number variation among varieties. We discuss implications of these genes existing on separate chromosomes (with homologs of each found in close proximity), and the significance of CBGAS being located on the X chromosome for cannabinoid production in female plants. This study provides valuable insight on the genomic identity and variation of cannabinoid biosynthesis genes that will benefit future research on the origin and evolution of this pathway, a driver of economic, social, and medicinal value.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
大麻素生物合成关键基因的基因组描述
大麻素生产是植物大麻的一个关键属性,表征所涉及的基因是开发优化工具必不可少的第一步。我们使用生物信息学方法来注释和探索大麻素途径中组成酶的基因编码的变化:来自不同谱系的多个大麻素基因组组装中的橄榄醇合成酶(OLS)、橄榄酸环化酶(OAC)、大麻酚酸合成酶(CBGAS)和大麻素氧化环化酶(THCAS、CBDAS、CBCAS)。前三种酶产生氧化环化酶的前体分子,以产生大麻素四氢大麻酚和CBD。结果表明,在不同品种中,OLS和OAC的重复序列是一致的,而基于系统发育比较,OAC的序列多样性最少。我们还发现一个cbgas样基因在品种间表现出拷贝数变异。我们讨论了这些基因存在于单独的染色体上的含义(每个染色体的同源物都很接近),以及CBGAS位于X染色体上对雌性植物大麻素产生的意义。这项研究为大麻素生物合成基因的基因组特征和变异提供了有价值的见解,这将有利于未来对这一途径的起源和进化的研究,这是经济、社会和医学价值的驱动因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Botany
Botany 生物-植物科学
CiteScore
2.20
自引率
9.10%
发文量
48
期刊介绍: Botany features comprehensive research articles and notes in all segments of plant sciences, including cell and molecular biology, ecology, mycology and plant-microbe interactions, phycology, physiology and biochemistry, structure and development, genetics, systematics, and phytogeography. It also publishes methods, commentary, and review articles on topics of current interest, contributed by internationally recognized scientists.
期刊最新文献
Four new species of Albomagister (Agaricales) from eastern North America The Meadoway: native meadow creation in underutilized transmission line corridors Do nurse plants and cattle exclusion help restore Parlatore’s Podocarp forest? Observations on the curvature of Physcomitrium patens (Hedw.) Mitt. and Funaria hygrometrica (Hedw.) caulonemal filaments 基于BP神经网络算法的延胡索农药减施增效技术研究
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1