Effects of different hemoglobin concentrations on lung injury in a rabbit model of hemorrhagic shock and resuscitation

Q4 Medicine 中华麻醉学杂志 Pub Date : 2019-10-20 DOI:10.3760/CMA.J.ISSN.0254-1416.2019.10.021

Xiao-Qian Liu, Li Liu, Xun Zhou, Na-Na Zhu, Yong-Quan Chen, Xiao-ju Jin, Jianrong Guo

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Abstract

Objective To evaluate the effect of different hemoglobin (Hb) concentrations on the lung injury in a rabbit model of hemorrhagic shock and resuscitation (HS-R). Methods Fifty healthy male New Zealand rabbits, aged 2 months, weighing 1.9-2.4 kg, were divided into 5 groups (n=10 each) using a random number table method: control group (group C), HS-R group (group H), and three HS-R plus infusion of concentrated red blood cell groups group R1 (60 g/L≤Hb<80 g/L), group R2 (80 g/L≤Hb<100 g/L) and group R3 (100 g/L≤Hb<120 g/L). The animals were sacrificed at 3 h after resuscitation, lung tissues were obtained for examination of the pathological changes with a light microscope, and lung tissues were obtained again for determination of wet to dry weight ratio (W/D ratio), neutrophil myeloperoxidase (MPO), NO level and cell apoptosis (by TUNEL). Results Compared with group C, the levels of MAP and NO, W/D ratio and apoptosis index were significantly increased in the other groups (P 0.05). Conclusion Maintaining Hb 80-100 g/L after HS-R reduces acute lung injury in rabbits. Key words: Shock, hemorrhagic; Cardiopulmonary resuscitation; Hemoglobins; Acute lung injury

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不同血红蛋白浓度对失血性休克及复苏兔肺损伤的影响

目的探讨不同血红蛋白(Hb)浓度对失血性休克复苏(HS-R)兔肺损伤的影响。方法50只2月龄、体重1.9 ~ 2.4 kg的健康雄性新西兰兔，采用随机数字表法分为5组(每组10只):对照组(C组)、HS-R组(H组)、HS-R加灌注浓缩红细胞组R1组(60 g/L≤Hb<80 g/L)、R2组(80 g/L≤Hb<100 g/L)、R3组(100 g/L≤Hb<120 g/L)。复苏后3 h处死大鼠，取肺组织光镜下观察病理变化，再次取肺组织测定干湿比(W/D ratio)、中性粒细胞髓过氧化物酶(MPO)、NO水平及细胞凋亡(TUNEL)。结果与C组比较，其余各组细胞内MAP、NO水平、W/D比值、凋亡指数均显著升高(P < 0.05)。结论HS-R术后维持Hb 80 ~ 100 g/L可减轻家兔急性肺损伤。关键词:休克;失血性;心肺复苏;血红蛋白;急性肺损伤

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