Min-Ji Park, Han-Cheol Lee, Ji-Young Yang, Jung-Beom Kim
{"title":"Development and validation of ultra-fast quantitative real-time PCR\n method to differentiate between Oncorhynchus keta and\n Oncorhynchus mykiss","authors":"Min-Ji Park, Han-Cheol Lee, Ji-Young Yang, Jung-Beom Kim","doi":"10.11002/kjfp.2023.30.3.383","DOIUrl":null,"url":null,"abstract":"\n \n The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was\n developed and validated to differentiate the morphologically similar ones,\n Oncorhynchus keta and Oncorhynchus mykiss.\n Species-specific primers were designed for the COI genes of\n mtDNA. The species-specific primers designed for O. keta and\n O. mykiss were selectively amplified by O.\n keta and O. mykiss DNA, respectively. The\n sensitivity of O. keta and O. mykiss primers\n was 1 ng/μL. Quantitative testing showed that the\n results met the ‘Guidelines on Standard Procedures for Preparing Analysis\n Method such as Food’ proposed by the Ministry of Food and Drug Safety.\n The qPCR method developed and validated in this study for identifying O.\n keta and O. mykiss has advantages such as speed\n and field applicability. Therefore, this method is expected to help control\n forgery and alteration of raw materials in the seafood industry.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2023.30.3.383","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was
developed and validated to differentiate the morphologically similar ones,
Oncorhynchus keta and Oncorhynchus mykiss.
Species-specific primers were designed for the COI genes of
mtDNA. The species-specific primers designed for O. keta and
O. mykiss were selectively amplified by O.
keta and O. mykiss DNA, respectively. The
sensitivity of O. keta and O. mykiss primers
was 1 ng/μL. Quantitative testing showed that the
results met the ‘Guidelines on Standard Procedures for Preparing Analysis
Method such as Food’ proposed by the Ministry of Food and Drug Safety.
The qPCR method developed and validated in this study for identifying O.
keta and O. mykiss has advantages such as speed
and field applicability. Therefore, this method is expected to help control
forgery and alteration of raw materials in the seafood industry.
期刊介绍：
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.
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