Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Entomological Science Pub Date : 2022-11-25 DOI:10.1111/ens.12526
Cheng-Ye Wang, Pan-Li Yang, Min Zhao, Huan-Li Xu, Li-Ning Liu, Ying Feng, Shi-Jie Wang
{"title":"Unusual mitochondrial tRNA rearrangements in stingless bee Tetragonula pagdeni and phylogenetic analysis","authors":"Cheng-Ye Wang,&nbsp;Pan-Li Yang,&nbsp;Min Zhao,&nbsp;Huan-Li Xu,&nbsp;Li-Ning Liu,&nbsp;Ying Feng,&nbsp;Shi-Jie Wang","doi":"10.1111/ens.12526","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Tetragonula pagdeni</i> (Hymenoptera: Meliponini) is a widely distributed and economically important stingless bee. The taxonomic status and evolution of this species have not been fully understood. We sequenced the mitogenome of <i>T</i>. <i>pagdeni</i> and analyzed its characteristics including gene rearrangements in present work, which will provide help for better protection and utilization of this germplasm resource. The mitochondrial genome sequence was obtained by establishing library and high-throughput sequencing. Using all protein coding sequences joined, the phylogenetic analysis demonstrated that <i>T</i>. <i>pagdeni</i> has the closest kinship with <i>Lepidotrigona</i> species, and then has a second closest kinship with the <i>Melipona</i> stingless bees. All the stingless bee species (Meliponini) analyzed formed a single clade, and among them the <i>T</i>. <i>pagdeni</i> mitogenome exhibited the highest gene rearrangement score, suggesting this species has an overactive evolutionary history. Especially, an unusual <i>tRNA-Gly</i> translocation was observed in <i>T</i>. <i>pagdeni</i> mitogenome, which broke the super-conserved <i>atp8-atp6-cox3-G</i> block in Hymenoptera mitogenomes. The translocated <i>tRNA-Gly</i> moved to a position next to the D-loop control region, and whether this translocation has a functional effect to the widely distributed <i>T</i>. <i>pagdeni</i> need further exploration. The variations of gene positions in mitogenome could also provide useful information to clarify the phylogenetic relations in Meliponini.</p>\n </div>","PeriodicalId":11745,"journal":{"name":"Entomological Science","volume":"25 4","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomological Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ens.12526","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Tetragonula pagdeni (Hymenoptera: Meliponini) is a widely distributed and economically important stingless bee. The taxonomic status and evolution of this species have not been fully understood. We sequenced the mitogenome of T. pagdeni and analyzed its characteristics including gene rearrangements in present work, which will provide help for better protection and utilization of this germplasm resource. The mitochondrial genome sequence was obtained by establishing library and high-throughput sequencing. Using all protein coding sequences joined, the phylogenetic analysis demonstrated that T. pagdeni has the closest kinship with Lepidotrigona species, and then has a second closest kinship with the Melipona stingless bees. All the stingless bee species (Meliponini) analyzed formed a single clade, and among them the T. pagdeni mitogenome exhibited the highest gene rearrangement score, suggesting this species has an overactive evolutionary history. Especially, an unusual tRNA-Gly translocation was observed in T. pagdeni mitogenome, which broke the super-conserved atp8-atp6-cox3-G block in Hymenoptera mitogenomes. The translocated tRNA-Gly moved to a position next to the D-loop control region, and whether this translocation has a functional effect to the widely distributed T. pagdeni need further exploration. The variations of gene positions in mitogenome could also provide useful information to clarify the phylogenetic relations in Meliponini.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
无刺蜂线粒体tRNA异常重排及其系统发育分析
帕格德尼四角蜂(膜翅目:Meliponini)是一种分布广泛、经济上重要的无刺蜂。该物种的分类地位和进化尚未完全了解。本工作对帕格德尼T.pagdeni的有丝分裂基因组进行了测序,并分析了其特征,包括基因重排,这将有助于更好地保护和利用该种质资源。通过建立文库和高通量测序获得线粒体基因组序列。使用所有连接的蛋白质编码序列,系统发育分析表明,T.pagdeni与鳞翅目物种的亲缘关系最密切,然后与Melipona无刺蜜蜂的亲缘关系第二密切。所有被分析的无刺蜜蜂物种(Meliponini)都形成了一个单一的分支,其中T.pagdeni有丝分裂基因组的基因重排得分最高,这表明该物种的进化史过于活跃。特别是,在P.pagdeni有丝分裂基因组中观察到一种不寻常的tRNA‐Gly易位,打破了膜翅目有丝分裂基因中超保守的atp8‐atp6‐cox3‐G区块。易位的tRNA‐Gly移到了D‐loop控制区旁边的位置,这种易位是否对广泛分布的T.pagdeni有功能影响需要进一步探索。有丝分裂基因组中基因位置的变化也可以为阐明Meliponini的系统发育关系提供有用的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Entomological Science
Entomological Science 生物-昆虫学
CiteScore
2.00
自引率
11.10%
发文量
30
审稿时长
6-12 weeks
期刊介绍: Entomological Science is the official English language journal of the Entomological Society of Japan. The Journal publishes original research papers and reviews from any entomological discipline or from directly allied field in ecology, behavioral biology, physiology, biochemistry, development, genetics, systematics, morphology, evolution and general entomology. Papers of applied entomology will be considered for publication if they significantly advance in the field of entomological science in the opinion of the Editors and Editorial Board.
期刊最新文献
A review of the genus Leptopa Zetterstedt, 1838 (Diptera: Scathophagidae) from Japan, with descriptions of two new species Immature stages and natural history of Eruga unilabiana (Hymenoptera: Ichneumonidae), an ectoparasitoid of two linyphiid spiders Finding a nest: Ant-associated silverfish (Zygentoma: Lepismatidae) moving outside granivorous Messor nests (Hymenoptera: Formicidae) Different habitat use of two Cybister (Coleoptera: Dytiscidae) species larvae in a paddy field water system Differences in sampling efficiency of each insect order in yellow pan traps with installation of flight interception windows
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1