Frequency of Extended-spectrum Beta-Lactamases-Producing Escherichia coli Among Out- and In-patients in Rafsanjan City, Iran

IF 0.5 4区 医学 Q4 MICROBIOLOGY Jundishapur Journal of Microbiology Pub Date : 2023-05-21 DOI:10.5812/jjm-133216
Ebrahim Rezazadeh Zarandi, H. Hakimi, R. Bahramabadi, M. Zare-Bidaki, M. Ramezani, Ali Shojaei, A. Khodayari, Maryam Fatemipour, Morteza Khademalhosseini, S. Assar
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Abstract

Background: The frequency of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains among clinical isolates has been steadily increasing, leading to limited treatment options. Objectives: This study aimed to assess the antibiotic sensitivity of E. coli and the frequency of ESBL isolates among both out-patients and in-patients. Methods: A total of 390 E. coli isolates were received at the Ali-Ebn-e-Abitaleb Hospital laboratory in Rafsanjan. The antibiogram, as well as the phenotypic and genotypic detection of ESBL isolates, were conducted using Kirby-Bauer, combination disk confirmatory, and polymerase chain reaction tests, respectively. Results: Of all the E. coli isolates, 45.6% exhibited ESBL production. Among these isolates, 46.1% were obtained from hospital wards, while 42.5% were from outpatients. Meropenem and imipenem displayed sensitivities of 97.2% and 93.3%, respectively, whereas amikacin and nitrofurantoin showed sensitivities of 89.7% and 85.6%, respectively, for all isolates. Notably, ceftriaxone, cefotaxime, cefixime, ampicillin, co-trimoxazole, and nalidixic acid demonstrated high resistance rates, surpassing 50%. ESBL-producing isolates were more frequently observed in blood samples (65%) and wounds (60%) compared to other tested isolates. Approximately 8.6% of isolates carried a single type of ESBL gene, while 38.5% carried multiple ESBL genes. Conclusions: The data indicate a prevalence of ESBL-producing E. coli isolates among both out-patients and in-patients, with some of them acquiring two or three types of ESBL enzymes. As a result, their ability to hydrolyze antibiotics has increased, leading to their higher occurrence in clinical samples.
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伊朗拉夫桑詹市门诊和住院患者中产生β -内酰胺酶的广谱大肠杆菌的频率
背景:临床分离株中产生超广谱β-内酰胺酶(ESBL)的大肠杆菌菌株的频率一直在稳步增加,导致治疗选择有限。目的:本研究旨在评估门诊和住院患者中大肠杆菌的抗生素敏感性和ESBL分离株的频率。方法:在拉夫桑詹Ali-Ebn-E-Abitaleb医院实验室共接收390株大肠杆菌分离株。分别使用Kirby-Bauer、组合圆盘验证和聚合酶链式反应测试进行抗体图谱以及ESBL分离株的表型和基因型检测。结果:在所有大肠杆菌分离株中,45.6%的菌株产ESBL。在这些分离株中,46.1%来自医院病房,42.5%来自门诊。美罗培南和亚胺培南对所有分离株的敏感度分别为97.2%和93.3%,而阿米卡星和呋喃妥因对所有分离物的敏感性分别为89.7%和85.6%。值得注意的是,头孢曲松、头孢噻肟、头孢克肟、氨苄青霉素、复方三恶唑和萘啶酸的耐药率较高,超过50%。与其他测试分离株相比,在血液样本(65%)和伤口(60%)中更频繁地观察到产生ESBL的分离株。大约8.6%的分离株携带单一类型的ESBL基因,而38.5%携带多个ESBL基因。结论:数据表明,在门诊患者和住院患者中,产生ESBL的大肠杆菌分离株普遍存在,其中一些人获得了两种或三种类型的ESBL酶。因此,它们水解抗生素的能力增加了,导致它们在临床样本中的发生率更高。
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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