COenzyme Q10 ameliorates bisphinol A induced reproductive male toxicity: A transmission electron microscopic and immunohistochemistry study

Samia S. Barghash, I. Farrag, Fatma El-Zahraa Abd El-Hakam, S. Ali, E. Aly
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Abstract

Background and aim The endocrine disruptor compound bisphenol A (BPA) affects spermatogenesis and exacerbates benign prostate hyperplasia induced by testosterone. Nonetheless, the direct effect of BPA on prostate and testicular cells is not fully investigated. The objective of this study was to evaluate the pathogenic effects of BPA on the structure of the prostate and the ultrastructure of the testis of adult male albino rats via immunohistochemical and transmission electron microscopic study and the potential protective effect of CoQ10 supplementation. Methods A total of 30 male albino Wistar rats were categorized into five equal cohorts: group I, no treatment; group II was administered corn oil; group III received coenzyme Q10 (CoQ10); group IV was administrated BPA; and group V received BPA+CoQ10. Results BPA administration significantly decreased the mean values of the plasma fertility hormones and serum antioxidant enzymes and increased malondialdehyde. BPA administration markedly affected seminal parameters. Coadministered CoQ10 significantly reversed these biochemical changes. BPA induced histopathological alterations in the epithelium and connective tissue of prostate. Immunohistochemistry of the prostate revealed decreased E-cadherin and increased vimentin expressions in BPA-treated group. Ultrastructural analysis of the testis showed impairment of the basal lamina of seminiferous tubules and tight junctions between Sertoli cells after BPA exposure. Conclusion The biochemical and histopathological results of this study revealed direct evidence for BPA-induced male reproductive toxicity in the testes and prostate, causing male infertility. CoQ10 coadministration with BPA partially protects against its damaging effect mediated via its antioxidant capabilities.
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辅酶Q10改善双酚A诱导的雄性生殖毒性:透射电镜和免疫组织化学研究
背景和目的内分泌干扰物双酚A(BPA)影响精子发生,并加剧睾酮诱导的良性前列腺增生。尽管如此,BPA对前列腺和睾丸细胞的直接影响尚未得到充分研究。本研究的目的是通过免疫组织化学和透射电镜研究,评估BPA对成年雄性白化大鼠前列腺结构和睾丸超微结构的致病作用,以及补充辅酶Q10的潜在保护作用。方法将30只雄性白化Wistar大鼠分为5组:第一组,不治疗;第二组给予玉米油;第三组给予辅酶Q10(CoQ10);IV组给予BPA;V组接受BPA+辅酶Q10。结果BPA能显著降低血浆生育激素和血清抗氧化酶的平均值,增加丙二醛含量。BPA给药显著影响精液参数。联合服用辅酶Q10可显著逆转这些生化变化。BPA引起前列腺上皮和结缔组织的组织病理学改变。前列腺免疫组织化学显示BPA治疗组E-钙粘蛋白表达减少,波形蛋白表达增加。睾丸的超微结构分析显示,BPA暴露后,曲精管基底层受损,支持细胞之间紧密连接。结论本研究的生化和组织病理学结果揭示了BPA在睾丸和前列腺中诱导男性生殖毒性,导致男性不育的直接证据。辅酶Q10与BPA的联合用药部分保护了其通过抗氧化能力介导的破坏作用。
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34 weeks
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