A Guide to Genome-Wide Association Mapping in Plants

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2017-03-03 DOI:10.1002/cppb.20041

Liana T. Burghardt, Nevin D. Young, Peter Tiffin

引用次数: 67

Abstract

Genome-wide association studies (GWAS) have developed into a valuable approach for identifying the genetic basis of phenotypic variation. In this article, we provide an overview of the design, analysis, and interpretation of GWAS. First, we present results from simulations that explore key elements of experimental design as well as considerations for collecting the relevant genomic and phenotypic data. Next, we outline current statistical methods and tools used for GWA analyses and discuss the inclusion of covariates to account for population structure and the interpretation of results. Given that many false positive associations will occur in any GWA analysis, we highlight strategies for prioritizing GWA candidates for further statistical and empirical validation. While focused on plants, the material we cover is also applicable to other systems. © 2017 by John Wiley & Sons, Inc.

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植物全基因组关联定位指南

全基因组关联研究(GWAS)已经发展成为鉴定表型变异遗传基础的一种有价值的方法。在本文中，我们概述了GWAS的设计、分析和解释。首先，我们展示了模拟结果，探索了实验设计的关键要素以及收集相关基因组和表型数据的考虑因素。接下来，我们概述了当前用于GWA分析的统计方法和工具，并讨论了协变量的纳入，以解释人口结构和结果的解释。鉴于在任何GWA分析中都会出现许多假阳性关联，我们强调了优先考虑GWA候选者的策略，以进一步进行统计和实证验证。虽然我们关注的是植物，但我们所涵盖的材料也适用于其他系统。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.