Raman spectroscopy: techniques and applications in the life sciences

IF 25.2 1区 物理与天体物理 Q1 OPTICS Advances in Optics and Photonics Pub Date : 2017-06-30 DOI:10.1364/AOP.9.000315
Dustin W. Shipp, F. Sinjab, I. Notingher
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引用次数: 178

Abstract

Raman spectroscopy is an increasingly popular technique in many areas, including biology and medicine. It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering, explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.
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拉曼光谱:技术及其在生命科学中的应用
拉曼光谱是一种越来越受欢迎的技术,在许多领域,包括生物和医学。它基于拉曼散射,这是一种入射光子通过与样品中振动分子的相互作用而失去或获得能量的现象。这些能量位移可用于获得有关样品分子组成的信息,准确度非常高。拉曼光谱在生命科学中的应用包括生物分子的定量、细胞和组织的高光谱分子成像、医学诊断等。本文简要介绍了拉曼散射的物理起源,解释了关键的经典和量子力学概念。拉曼效应的变化也将被考虑,包括共振、相干和增强拉曼散射。我们讨论了在生物样品的拉曼光谱中经常发现的突出波段的分子起源。最后,我们研究了拉曼光谱技术在实践中的几种变化,研究了它们的应用、优势和挑战。本文旨在为新接触拉曼光谱的科学家提供一个入门资源,为进一步的研究和探索提供理论背景和实际例子。
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来源期刊
CiteScore
56.60
自引率
0.00%
发文量
13
期刊介绍: Advances in Optics and Photonics (AOP) is an all-electronic journal that publishes comprehensive review articles and multimedia tutorials. It is suitable for students, researchers, faculty, business professionals, and engineers interested in optics and photonics. The content of the journal covers advancements in these fields, ranging from fundamental science to engineering applications. The journal aims to capture the most significant developments in optics and photonics. It achieves this through long review articles and comprehensive tutorials written by prominent and respected authors who are at the forefront of their fields. The journal goes beyond traditional text-based articles by enhancing the content with multimedia elements, such as animation and video. This multimedia approach helps to enhance the understanding and visualization of complex concepts. AOP offers dedicated article preparation and peer-review support to assist authors throughout the publication process. This support ensures that the articles meet the journal's standards and are well-received by readers. Additionally, AOP welcomes comments on published review articles, encouraging further discussions and insights from the scientific community. In summary, Advances in Optics and Photonics is a comprehensive journal that provides authoritative and accessible content on advancements in optics and photonics. With its diverse range of articles, multimedia enhancements, and dedicated support, AOP serves as a valuable resource for professionals and researchers in these fields.
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