Genetic Assessment of Some Egyptian Cultivated Citrus and its Relatives using Retrotransposons and Microsatellite

Abstract

detailed overview of evaluating 21 LTR-REMAP and 27 SSR markers in the discriminating capacity, efficiency and ability of the genetic variability among 20 genotypes in the genus Citrus . The LTR-REMAP profile displayed 1.28 fold higher value of marker index (MI) compare to the SSR profile, highlights the distinctive nature of LTR-REMAP assay. This marker was more sensitive and provided much more evidence to discriminate at low taxonomic levels, especially for Egyptian acidic, whereas, SSR can differentiate within the group level only. Intriguingly, the heatmap cluster analysis (HCA) and principal component analysis (PCA) data drew an accurate monophyletic annotations cluster in Citreneae species, offer unambiguous identification without overlapping groups between true species and related hybrids of citrus, resulted in their placement in different clades. The use of molecular technologies will help to overcome the obstacles of cultivars identification, genetic variability in the Egyptian citrus breeding.