Insecticidal effect of the entomopathogenic nematode Heterorhabditis megidis (Nematoda: Heterorhabditidae) baited from the soil on the larvae of Monochamus alternatus (Coleoptera: Cerambycidae)
Sota Ozawa, Noritoshi Maehara, Jun Takatsuka, Takuya Aikawa, Katsunori Nakamura
{"title":"Insecticidal effect of the entomopathogenic nematode Heterorhabditis megidis (Nematoda: Heterorhabditidae) baited from the soil on the larvae of Monochamus alternatus (Coleoptera: Cerambycidae)","authors":"Sota Ozawa, Noritoshi Maehara, Jun Takatsuka, Takuya Aikawa, Katsunori Nakamura","doi":"10.1007/s13355-023-00820-1","DOIUrl":null,"url":null,"abstract":"<div><p>To scout for an entomopathogenic nematode (EPN) that effectively controls the pine sawyer beetle, <i>Monochamus alternatus</i> Hope (Coleoptera: Cerambycidae), we screened EPNs from soil samples using <i>M. alternatus</i> larvae as bait. A population of the nematode obtained was named isolate SOz01, and molecularly identified as <i>Heterorhabditis megidis</i> Poinar, Jackson and Klein (Nematoda: Heterorhabditidae). In the inoculation test of the infective juveniles (IJs) of SOz01 onto mature larvae of <i>M. alternatus</i> under laboratory conditions, a mortality of 86–100% occurred when more than 20 IJs were inoculated. Even when the larvae were inoculated with only five IJs, one-third of them died. This finding suggests that <i>H. megidis</i> SOz01 has a strong insecticidal effect on <i>M. alternatus</i>.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 2","pages":"197 - 203"},"PeriodicalIF":1.3000,"publicationDate":"2023-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00820-1.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Entomology and Zoology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13355-023-00820-1","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
To scout for an entomopathogenic nematode (EPN) that effectively controls the pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae), we screened EPNs from soil samples using M. alternatus larvae as bait. A population of the nematode obtained was named isolate SOz01, and molecularly identified as Heterorhabditis megidis Poinar, Jackson and Klein (Nematoda: Heterorhabditidae). In the inoculation test of the infective juveniles (IJs) of SOz01 onto mature larvae of M. alternatus under laboratory conditions, a mortality of 86–100% occurred when more than 20 IJs were inoculated. Even when the larvae were inoculated with only five IJs, one-third of them died. This finding suggests that H. megidis SOz01 has a strong insecticidal effect on M. alternatus.
为了寻找一种能有效控制松毛虫的昆虫病原线虫(Monochamus alternatus Hope)(鞘翅目:天牛科)，我们在土壤样品中筛选了一种能有效控制松毛虫的昆虫病原线虫(EPN)。获得的线虫种群命名为SOz01分离株，分子鉴定为异habditis megidis Poinar, Jackson and Klein(线虫纲:异habditidae)。在实验室条件下，将侵染SOz01的幼虫接种于交替田鼠成熟幼虫上，接种20只以上，死亡率为86-100%。即使幼虫只接种了5只ij，也有三分之一的幼虫死亡。这一发现表明，megidis SOz01对交替田鼠有较强的杀虫作用。
期刊介绍：
Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
