Repairing rat sciatic nerve defects via transplanting bilayer collagen conduit scaffolds carrying bone marrow mesenchymal stem cells

Ximeng Ji, H. Zhuang, Yiwen Zhang, Jincan Zhang, Bu Shoushan
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Abstract

Objective To construct an artificial nerve graft as an alternative for autologous nerve grafting. Methods We fabricated an innovative tissue engineered nerve graft (TENG), which consists of a commercial bilayer collagen membrane (Bio-Gide)-based nerve scaffold and bone marrow mesenchymal stem cells (BMSCs) serving as support cells to bridge a 10 mm long sciatic nerve defect in rats, The 10 mm gap was left empty after resection in the blank group. In the other three groups, the sciatic nerve defect was, respectively, bridged with plain collagen tube (CT group), a collagen tube filled with BMSC suspension (C+ CT group), or reversed transected nerve segment (AG group). Results Histological and functional assessments showed that the developed TENG facilitated nerve regeneration (SFI: CT, -92.490±1.836; C+ CT, -70.010±7.805; AG, -70.130±8.744; F= 17.850, P 0.05) and better outcomes than the plain collagen tube (P<0.05). Conclusion These results manifested that the bilayer collagen conduit loaded BMSCs could be applied as a new biodegradable artificial nerve guide for nerve tissue engineering. Key words: Tissue engineer; Nerve graft; Bone marrow mesenchymal stem cells; Bilayer collagen conduit scaffold; Sciatic nerve injury; Peripheral nerve regeneration
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骨髓间充质干细胞双层胶原导管支架移植修复大鼠坐骨神经缺损
目的构建人工神经移植物作为自体神经移植的替代方法。方法利用商业双层胶原膜(Bio-Gide)神经支架和骨髓间充质干细胞(BMSCs)作为支撑细胞,构建新型组织工程神经移植物(TENG),用于大鼠坐骨神经缺损的修复,空白组切除后留下10 mm的缺损。其余三组分别用胶原蛋白管(CT组)、胶原蛋白管填充BMSC悬浮液(C+ CT组)或反截神经段(AG组)桥接坐骨神经缺损。结果组织学和功能评估显示,发育的TENG促进了神经再生(SFI: CT, -92.490±1.836;C+ ct, -70.010±7.805;AG), -70.130±8.744;F= 17.850, P 0.05),且效果优于普通胶原管(P<0.05)。结论双层胶原导管载骨髓间充质干细胞可作为一种新型的生物可降解人工神经引导材料应用于神经组织工程。关键词:组织工程师;神经移植;骨髓间充质干细胞;双层胶原导管支架;坐骨神经损伤;周围神经再生
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