Cloning and expression analysis of ZjHSP18.9 from Zoysia japonica

IF 1.1 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Grassland Science Pub Date : 2022-02-21 DOI:10.1111/grs.12359

Zhi-yong Huang, Xin-bo Sun, Yao Zou

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Abstract

The ZjHSP18.9 gene was isolated from Zoysiagrass by homologous cloning based on publicly available transcriptome database. Sequence alignment analysis revealed that ZjHSP18.9 protein contains a classic Hsp23/ACD domain. Phylogenetic analysis and subcellular localization prediction suggested that ZjHSP18.9 belongs to cytosolic class VI subfamily. The expression profiles of ZjHSP18.9 in leaf and root after abiotic stress or Abscisic Acid (ABA) treatment was investigated using quantitative real-time polymerase chain reaction. The results showed that the expression level of ZjHSP18.9 in leaf was higher than that in root. The expression of ZjHSP18.9 was strongly induced by high temperature in leaf; the ZjHSP18.9 expression level was up-regulated in both leaf and root by the treatment of low temperature, salt and heavy metal, while down-regulated under the treatment of drought and ABA. The gene expression profiles indicated that ZjHSP18.9 may play an important role in Zoysiagrass response to environmental stress including extreme temperature, salinity, water deficiency and heavy metal through ABA dependent and/or independent pathway.

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结缕草ZjHSP18.9基因的克隆与表达分析

利用公开转录组数据库同源克隆从结缕草中分离到ZjHSP18.9基因。序列比对分析显示，ZjHSP18.9蛋白含有一个典型的Hsp23/ACD结构域。系统发育分析和亚细胞定位预测表明，ZjHSP18.9属于细胞质VI类亚家族。采用实时荧光定量聚合酶链反应(pcr)研究了非生物胁迫和ABA处理后ZjHSP18.9在叶片和根中的表达谱。结果表明，ZjHSP18.9在叶片中的表达量高于根中的表达量。高温对ZjHSP18.9的表达有强烈的诱导作用;ZjHSP18.9的表达量在低温、盐和重金属处理下均上调，在干旱和ABA处理下均下调。基因表达谱表明，ZjHSP18.9可能通过ABA依赖和/或独立途径在结草对极端温度、盐度、缺水和重金属等环境胁迫的响应中发挥重要作用。

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来源期刊

Grassland Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields: grassland environment, landscape, ecology and systems analysis; pasture and lawn establishment, management and cultivation; grassland utilization, animal management, behavior, nutrition and production; forage conservation, processing, storage, utilization and nutritive value; physiology, morphology, pathology and entomology of plants; breeding and genetics; physicochemical property of soil, soil animals and microorganisms and plant nutrition; economics in grassland systems.

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