Biomass Production of Microalgae using Agricultural and Industrial Wastewater

Abstract

Growing wastewater microalgae contributes to the elimination of nutrients and pollutants present in wastewater because they need N and P for the synthesis of proteins, nucleic acid, and phospholipids. The most sustainable approach for achieving high biomass and high lipid build-up, along with environmental protection. In the current research, nine microalgae were cultivated on two variations of wastewater (agricultural and industrial dyes wastewater) and compared to the synthetic medium. The results indicated that after three weeks of the incubation period, the ability of microalgae to grow in sterilized synthetic medium and wastewater and gave greater biomass and chlorophyll (a) than in non-sterilized ones. Out of the tested nine microalgae, four microalgae (Nostoc muscorum, Anabeana oryzae, Spirulina platensis and Anabeana fertilissima) were selected which gave the highest significant values of dry weight, biomass productivity and chlorophyll (a) content. The selected microalgae showed the highest significant values of the chemical composition, i.e. total carbohydrate, protein and lipid when cultivated in agricultural wastewater more than those cultivated in both industrial dyes wastewater and synthetic broth medium. Among four microalgae, two microalgae of A. oryzae HSSASE6 (KT277789), and S. platensis NIES-39 (A00800) were chosen where the chemical composition contents ranged from 1.17 to 1.21-fold and 1.03 to 1.06-fold of carbohydrate, 1.06 to 1.09-fold and 1.88 to 1.93-fold of protein and 1.41 to 1.52-fold and 1.76 to 1.90-fold of lipid more than those of other microalgae, respectively. The agriculture wastewater was inoculated with a single culture of A. oryzae HSSASE6 (KT277789) or S. platensis NIES-39 (A00800) individually with 10 % of inoculum size, which more preferred than was inoculated with consortia culture. Results also showed that A. oryzae HSSASE6 (KT277789) was more efficient strain for giving biomass and productivity in agricultural wastewater than S. platensis NIES-39 (A00800) (about 28% more ).