An Early Molecular Biomarker Study in Pre-eclampsia

Abstract

Preeclampsia (PE), a hypertension condition associated with pregnancy that manifests after 20 weeks of pregnancy,may be brought on by faulty placental development and poses a serious risk to both the mother and the fetus. The disease can bedetected only once the symptoms arise, there is a need for a biomarker, which makes early detection of the disease possible, andproper management of the disease can be done at an early stage of pregnancy. Even though many studies were done globallyregarding the role of microRNAs as molecular biomarkers in pre-eclampsia, no literature showed the effect of microRNA inpreeclampsia in the Indian population. The aim of the study was to find an early molecular marker that can detect the disease atan early stage, even before the symptoms arise. Placental MicroRNAs were studied using Next-generation Sequencing of 60Preeclamptic groups ( 30 Early Onset,30Late onset preeclampsia), and 30 control groups were selected; miRNA profiling wasdone by Illumina sequencing, and the quantifier did downstream analysis for identification, quantification, and expression profiling.pl, script, and miRDeep2.pl, script. Total RNAs were extracted from placental tissues and cells by TRIzol reagent and purified. Therelative expression of miR-483-5p in tissues or cells was determined: micro-RNA 483-5p was expressed in significant quantity inEarly Onset preeclamptic placental samples compared to Late Onset and normal samples. miR 483-5p was analyzed, and the genetargets were found using computational methods. From this study, it was found that microRNA 483-5p can be used as an earlybiomarker for the identification of preeclampsia.