{"title":"Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection","authors":"R. Liu","doi":"10.46770/as.2021.1013","DOIUrl":null,"url":null,"abstract":": The precise targeting and excellent specific cleave capability of CRISPR/Cas (clustered regularly inters-paced short palindromic repeats-associated proteins) have gained much research interest. In addition to nucleic acid detection, researchers exploring the potential of this system for detecting various other targets. Herein, we proposed a novel CRISPR/Cas biosensing based Tobramycin (TOB) detection method. Combining with the aptamer recognition and element labeling strategy, highly sensitive and specific detection of TOB is realized. Due to the powerful elemental analysis capabilities of inductively coupled plasma mass spectrometry (ICP-MS) and the excellent signal amplification effect of CRISPR/Cas, the linear concentration range for TOB was 2.8-100 nM in this method, whereas the detection limit was 0.83 nM. The proposed method has been successfully applied in the analysis of actual water samples.","PeriodicalId":8642,"journal":{"name":"Atomic Spectroscopy","volume":" ","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Atomic Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.46770/as.2021.1013","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
引用次数: 2
Abstract
: The precise targeting and excellent specific cleave capability of CRISPR/Cas (clustered regularly inters-paced short palindromic repeats-associated proteins) have gained much research interest. In addition to nucleic acid detection, researchers exploring the potential of this system for detecting various other targets. Herein, we proposed a novel CRISPR/Cas biosensing based Tobramycin (TOB) detection method. Combining with the aptamer recognition and element labeling strategy, highly sensitive and specific detection of TOB is realized. Due to the powerful elemental analysis capabilities of inductively coupled plasma mass spectrometry (ICP-MS) and the excellent signal amplification effect of CRISPR/Cas, the linear concentration range for TOB was 2.8-100 nM in this method, whereas the detection limit was 0.83 nM. The proposed method has been successfully applied in the analysis of actual water samples.
期刊介绍：
The ATOMIC SPECTROSCOPY is a peer-reviewed international journal started in 1962 by Dr. Walter Slavin and now is published by Atomic Spectroscopy Press Limited (ASPL). It is intended for the rapid publication of both original articles and review articles in the fields of AAS, AFS, ICP-OES, ICP-MS, GD-MS, TIMS, SIMS, AMS, LIBS, XRF and related techniques. Manuscripts dealing with (i) instrumentation & fundamentals, (ii) methodology development & applications, and (iii) standard reference materials (SRMs) development can be submitted for publication.
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