Effects of thermal stress caused by the 2015–2016 El Niño on the biochemical composition, exoskeleton structure, and symbiont density of the fire coral Millepora alcicornis

Abstract

Reef-forming cnidarians are essential for maintaining ecological balance. Unfortunately, coral reefs are endangered due to coral bleaching, which interrupts mutualistic symbiosis between Symbiodiniaceae algae and their coral hosts. Bleaching events result in very high coral mortality and the rapid deterioration of reef structures. Studies aimed at explaining the causes, mechanisms, and consequences of coral bleaching have been mainly conducted with anthozoans, while the impacts of thermal stress responsible for coral bleaching have been scarcely studied in hydrozoans, such as Millepora species (phylum Cnidaria, class Hydrozoa), which are the second most important reef-forming cnidarians. In the present study, the effects of thermal stress caused by the 2015–2016 El Niño on symbiont abundance, exoskeleton structure, and the biochemical composition of Millepora alcicornis were analyzed. Unbleached M. alcicornis specimens exhibited a higher abundance of Breviolum and Durisdinium species, which suggests that unbleached hydrocoral colonies might counteract thermal stress by hosting thermotolerant symbionts of the Durisdinium genus. Bleached hydrocorals exhibited lower levels of calcification than unbleached hydrocorals as well as changes in the microstructure of trabeculae and zooid pores. In contrast, thermal stress did not affect total calcium carbonate and carbohydrate content. Bleached tissues showed significantly higher levels of protein and refractory material, whereas their lipid content decreased considerably. The present study provides evidence that bleached M. alcicornis colonies suffered a decline in calcification and changes in the structure of their exoskeletons after being exposed to the 2015–2016 El Niño. The significant decrease in lipid content suggests that M. alcicornis primarily uses energy stores to maintain vital cellular processes at the expense of calcification.