Naila Chaabna, Oumaima Naili, Nafissa Ziane, C. Bensouici, S. Dahamna, D. Harzallah
{"title":"Neuroprotective and Antioxidant Activities of Algerian Punica granatum L. Peel Extracts","authors":"Naila Chaabna, Oumaima Naili, Nafissa Ziane, C. Bensouici, S. Dahamna, D. Harzallah","doi":"10.2174/1573408019666230626142826","DOIUrl":null,"url":null,"abstract":"\n\nOxidative stress is implicated in many neurodegenerative disorders, such as Alzheimer’s disease. As an alternative, Punica granatum peels are widely used in the treatment of several ailments. To date, this is the first study on the anti-acetyl/butyrylcholinesterase effects of diethyl ether extract from Algerian pomegranate peels.\n\n\n\nThis research aimed to evaluate the content of total phenolics, flavonoids, flavonols, and tannins in Punica granatum peel crude extract and its fraction diethyl ether, as well as to assess its antioxidant and neuroprotective activities.\n\n\n\nThe total phenolic, flavonoid, flavonol, and tannin contents in the extracts were evaluated using spectrophotometric methods. The inhibitory effects on acetyl/butyrylcholinesterase were assessed using the in vitro models. In addition, several methods were used to investigate the antioxidant capacity of extracts, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) free radicals scavenging, phenanthroline, reducing power and cupric ion reducing antioxidant capacity (CUPRAC) assays.\n\n\n\nPhytochemical analysis revealed that the diethyl ether extract recorded the highest content of total phenolics, flavonoids, flavonols, and tannins with values of 643.3±9.96µgGAE/mg, 78.97±9.97µgQE/mg, 31.32±1.79µgQE/mg and 96.6±13.65µgTAE/mg, respectively. Moreover, the diethyl ether extract exhibited the strongest activity in the DPPH scavenging assay (IC50=3.36±0.11µg/mL), ABTS scavenging assay (IC50=1.46±0.10µg/mL), CUPRAC assay (CA0.5=3.62±0.36µg/mL), phenanthroline assay (CA0.5=1.04±0.10µg/mL), reducing power assay (CA0.5=4.14±0.32µg/mL), anti-acetylcholinesterase assay (IC50=114.86±13.66µg/mL), and anti-butyrylcholinesterase assay (IC50=147.00±10.41µg/mL).\n\n\n\nThe present study has shown the extracts from Punica granatum peel to be a rich source of bioactive compounds, potent antioxidants, and cholinesterase inhibitors. They could be considered as natural agents that can be used in anti-neurodegenerative pharmaceutical formulations.\n","PeriodicalId":35405,"journal":{"name":"Current Enzyme Inhibition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Enzyme Inhibition","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1573408019666230626142826","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0
Abstract
Oxidative stress is implicated in many neurodegenerative disorders, such as Alzheimer’s disease. As an alternative, Punica granatum peels are widely used in the treatment of several ailments. To date, this is the first study on the anti-acetyl/butyrylcholinesterase effects of diethyl ether extract from Algerian pomegranate peels.
This research aimed to evaluate the content of total phenolics, flavonoids, flavonols, and tannins in Punica granatum peel crude extract and its fraction diethyl ether, as well as to assess its antioxidant and neuroprotective activities.
The total phenolic, flavonoid, flavonol, and tannin contents in the extracts were evaluated using spectrophotometric methods. The inhibitory effects on acetyl/butyrylcholinesterase were assessed using the in vitro models. In addition, several methods were used to investigate the antioxidant capacity of extracts, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) free radicals scavenging, phenanthroline, reducing power and cupric ion reducing antioxidant capacity (CUPRAC) assays.
Phytochemical analysis revealed that the diethyl ether extract recorded the highest content of total phenolics, flavonoids, flavonols, and tannins with values of 643.3±9.96µgGAE/mg, 78.97±9.97µgQE/mg, 31.32±1.79µgQE/mg and 96.6±13.65µgTAE/mg, respectively. Moreover, the diethyl ether extract exhibited the strongest activity in the DPPH scavenging assay (IC50=3.36±0.11µg/mL), ABTS scavenging assay (IC50=1.46±0.10µg/mL), CUPRAC assay (CA0.5=3.62±0.36µg/mL), phenanthroline assay (CA0.5=1.04±0.10µg/mL), reducing power assay (CA0.5=4.14±0.32µg/mL), anti-acetylcholinesterase assay (IC50=114.86±13.66µg/mL), and anti-butyrylcholinesterase assay (IC50=147.00±10.41µg/mL).
The present study has shown the extracts from Punica granatum peel to be a rich source of bioactive compounds, potent antioxidants, and cholinesterase inhibitors. They could be considered as natural agents that can be used in anti-neurodegenerative pharmaceutical formulations.
期刊介绍：
Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.
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