S. Al-Muhanna, Israa Abdul Ameer Al-Kraety, S. Banoon
{"title":"Molecular detection of spa gene among Staphylococcus aureus isolated from mastitis","authors":"S. Al-Muhanna, Israa Abdul Ameer Al-Kraety, S. Banoon","doi":"10.22034/IJI.V8I0.589","DOIUrl":null,"url":null,"abstract":"From June to September 2020, among thirty gram-positive bacteria grown with mannitol salt agar, seventeen Staphylococcus aureus isolates were described. Staphylococcus aureus isolates were described in the initial identification based on the colonial morphology, microscopic analysis, and biochemical tests. The final identification was performed using an automated VITEK-2 compact system. The spa genes were detected by the genotyping method using the polymerase chain reaction (PCR) technique. The results showed that the spa gene comprised 19 isolates (63.3%) of S. aureus .","PeriodicalId":14599,"journal":{"name":"Iranian Journal of Ichthyology","volume":"8 1","pages":"16-20"},"PeriodicalIF":0.0000,"publicationDate":"2021-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Ichthyology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22034/IJI.V8I0.589","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
From June to September 2020, among thirty gram-positive bacteria grown with mannitol salt agar, seventeen Staphylococcus aureus isolates were described. Staphylococcus aureus isolates were described in the initial identification based on the colonial morphology, microscopic analysis, and biochemical tests. The final identification was performed using an automated VITEK-2 compact system. The spa genes were detected by the genotyping method using the polymerase chain reaction (PCR) technique. The results showed that the spa gene comprised 19 isolates (63.3%) of S. aureus .
期刊介绍：
Iranian Journal of Ichthyology (IJI) is a peer-reviewed journal for publication of high quality papers on any aspect of ichthyology and will be published 4 times a year by the Iranian Society of Ichthyology (http://www.isi-org.ir). The journal welcomes the submission of manuscripts that meets the general criteria of significance and scientific excellence, and will publish original scientific articles in the fields of ichthyology. The editors welcome the submission of original manuscripts including Research Articles, Short communications, and Views & News. Review papers by distinguished authors and Special Issues will also be considered. It is our aim to provide the international scientific community with an efficiently published journal, meeting high scientific and technical standards. Scopes of Iranian Journal of Ichthyology includes: Systematics, taxonomy, morphology, anatomy, biogeography, biodiversity, conservation, ecology and general biology of fishes (reproduction, food and feeding habits, age and growth, population dynamics, etc.). Papers on freshwater, brackish, marine and fossil fishes will be considered.
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