Screening of Antimicrobial, Antibiofilm, and Cytotoxic Activities of Some Medicinal Plants from Balıkesir Province, Türkiye: Potential Effects of Allium paniculatum Flower.

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-07-12 DOI:10.4274/tjps.galenos.2023.88935

Özlem Oyardı, Mayram Hacıoğlu, Ebru Özdemir, Meryem Şeyda Erbay, Şükran Kültür, Çağla Bozkurt Güzel

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Abstract

Objectives: Plant extracts are important natural resources that may have antimicrobial and antibiofilm effects against pathogens. This study was conducted to investigate the in vitro antimicrobial activities of methanol extracts of some medicinal plants (Achillea nobilis subspecies neilreichii (A. Kern.) Velen., Aetheorhiza bulbosa (L.) Cass, Allium paniculatum L, Asphodelus aestivus Brot., Ballota nigra L., Cistus laurifolius L., Cistus salviifolius L., Dioscorea communis (L.) Caddick and Wilkin, Galium verum L., Hypericum triquetrifolium Turra, Paliurus spina-christi Mill., Primula vulgaris Huds. subspecies rubra (Sm.) Arcang., Ranunculus arvensis L. and Teucrium polium L.) from Balıkesir province in Türkiye.

Materials and methods: Preliminary antimicrobial activity screening was conducted for all extracts. Antibiofilm activity studies were conducted on mature Candida albicans biofilms. Moreover, the cytotoxicities of A. paniculatum flower extract on A549 and Vero cell lines were determined using a colorimetric tetrazolium-based assay.

Results: A. paniculatum flower, P. vulgaris root, C. laurifolius, C. salviifolius, and A. nobilis displayed good activity [minimum inhibitory concentrations (MIC): 9.75, 156, 312, 312 and 312 μg/mL, respectively] against C. albicans American Type Culture Collection 10231. Biofilm studies were conducted on these plant extracts. The methanol extract of A. paniculatum flower decreased the number of C. albicans [colony-forming unit (CFU)/mL] in mature biofilm statistically at 32 x MIC and higher concentrations (p < 0.01). A. paniculatum flower extract had a cytotoxic effect (killing more than 50% of cells) at high concentrations, and its effect on Vero cells was similar to that on A549 cells.

Conclusion: This study demonstrated the importance of the methanol extract of A. paniculatum flower as a natural alternative against C. albicans infections, including biofilms.

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土耳其Balıkesir省部分药用植物的抗菌、抗菌膜和细胞毒性筛选：指出葱花的潜在作用

目的：植物提取物是一种重要的天然资源，可能对病原体具有抗菌和抗生物膜作用。本研究旨在研究一些药用植物（Achillea nobilis L.subsp.neilreichii（A.Kern.）Velen。，球叶Aetheohiza bulbosa（L.）Cass，葱（Allium paniculatum L），普通小麦（Aspodelus aestivus Brot.）。，Ballota nigra L.、Cistus laurifolius L.、Cistos salviifolius L.、Dioscorea consus（L.）Caddick&Wilkin、Galium verum L.、Hypericum triquetrifolium Turra、Palius spina christi Mill。，寻常报春花。亚种。rubra（Sm.）Arcing。，Ranunculus arvensis L.和Teucrium polium L.）。材料与方法：对所有提取物进行初步抗菌活性筛选。对白色念珠菌成熟生物膜进行了抗菌膜活性研究。此外，使用基于比色四氮唑的测定法测定了A.paniculatum花提取物对A549和Vero细胞系的细胞毒性。结果：A.paniculatum花、P.vulgaris根、C.laurifolius、C.salvifolius和A.nobilis对白色念珠菌ATCC 10231表现出良好的活性（MICs分别为9.75、156、312、312和312µg/ml）。对这些植物提取物进行了生物膜研究。在32xMIC及更高浓度下，圆锥花甲醇提取物可显著降低成熟生物膜中白色念珠菌的数量（cfu/ml）（P<0.01）。圆锥花甲醇提取液在高浓度下具有细胞毒性作用（杀死50%以上的细胞），对Vero细胞的作用与对A549细胞的作用相似。结论：本研究证明了圆锥花甲醇提取物作为包括生物膜在内的白色念珠菌感染的天然替代品的重要性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.