Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies

Abstract

Mitochondria are providing an essential amount of  energy to the cell, to achieve in homeostasis, metabolic  increases, proliferation and differentiation processes.  Also, mitochondrial deficiencies have severe or lethal  impacts on cell viability. Among the 3000 proteins  involved in mitochondrial activities, ATAD3 is a major  one as essential for mitochondrial biogenesis, vital as  early as embryonic implantation.  In order to see its  impact at animal level, we have  used ATAD3+/- mice to investigate its role in running  training and in high calorie diet.  We found here that ATAD3 expression level  avoids running capacity improvement and has a strong  effect on weight increase, underlying its important role  in mitochondrial mass regulations.  Prior to this presentation we will emphasize on the  potential of Western-blot, PCR and  immunofluorescence analysis in biomedical researches.