Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extract (FSA)

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL International Medical Journal Malaysia Pub Date : 2020-11-09 DOI:10.31436/IMJM.V15I1.410
Ahmed Kaid N. Allaw, Norbaiyah Mb, Norazian Mh
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引用次数: 5

Abstract

Introduction: This study aims to build a standardization method for preparation of effective powder from FSA and to quantify diosgenin in FSA. Methodology: One kg of FS were used in this study. Setting: BMS, KOM and KOP, IIUM Kuantan campus. FS were washed with distilled water to exclude any foreign matter, and were then air dried. FS-powder were put in distilled water in a ratio of 1 g of powder in 20 ml of distilled water and were shaken at room temperature for 24 hours. Ten mg of hydrolyzed extract sample was diluted in 10 ml volumetric flask with methanol for 15 minutes. Chromatographic estimation was performed using an equilibrated reverse phase Eclipse XDB-C18 column (particle size 5 μg, 4.6 mm x 150 mm). Results: One gram of FSA extract was hydrolyzed to produce sapogenins and 46.6% was recovered. A calibration curve that was constructed based on five dilutions of diosgenin standard at concentrations of 2, 5, 10, 20, 30 and 50 ppm produced a linear graft (r = 0.999). The concentration of diosgenin in FSA extract as calculated using the regression analysis was found to be 29.66 μg/ml, 13.81 % w/w on dried weight basis. Conclusion: Preparation and standardization of effective powder from FSA are the corner stone of many scientific researches in IIUM and Malaysia. Diosgenin is available in the FSA in adequate concentration. The adequate amount of diosgenin in the FSA will guide us to do further study in the way of preparation of a natural product that can be used in the field of reversible anti-fertility therapy.
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胡芦巴种子水提物中抗生育化合物薯蓣皂苷元的含量测定
前言:本研究旨在建立黄芪皂苷元制备有效粉末的标准化方法,并定量测定黄芪皂苷元的含量。方法:本研究采用FS 1 kg。地点:BMS, KOM和KOP, IIUM关丹校区。FS用蒸馏水清洗以排除任何异物,然后风干。将fs粉按1g粉与20ml蒸馏水的比例放入蒸馏水中,室温下摇匀24小时。将10mg水解提取液样品用甲醇在10ml容量瓶中稀释15min。色谱估计采用平衡反相Eclipse XDB-C18色谱柱(粒径5 μg, 4.6 mm x 150 mm)。结果:黄芪提取物1克水解得到皂苷元,回收率为46.6%。在浓度为2、5、10、20、30和50 ppm的5种稀释条件下,建立了一种线性接枝曲线(r = 0.999)。经回归分析,黄芪提取物中薯蓣皂苷元的含量为29.66 μg/ml,按干重计为13.81% w/w。结论:FSA有效粉末的制备和标准化是IIUM和马来西亚许多科学研究的基石。薯蓣皂苷元在食品标准局有足够的浓度。FSA中薯蓣皂苷元的充足含量将指导我们在制备一种可用于可逆抗生育治疗领域的天然产品的方式上做进一步的研究。
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来源期刊
International Medical Journal Malaysia
International Medical Journal Malaysia Medicine-Medicine (all)
CiteScore
0.40
自引率
0.00%
发文量
73
期刊介绍: International Medical Journal Malaysia (IMJM) is the official journal of the Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia. It serves primarily as a forum for education and intellectual discourse for health professionals namely in clinical medicine but covers diverse issues relating to medical ethics, professionalism as well as medical developments and research in basic medical sciences. It also serves the unique purpose of highlighting issues and research pertaining to the Muslim world. Contributions to the IMJM reflect its international and multidisciplinary readership and include current thinking across a range of specialties, ethnicities and societies.
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