Comparison of Changes in the Content of Plasma Membrane and Tonoplast Sterols under Oxidative and Osmotic Stress

N. V. Ozolina, V. V. Gurina, I. S. Kapustina, E. V. Spiridonova, V. N. Nurminsky
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引用次数: 1

Abstract

We compared oxidative and osmotic stress-induced changes in sterol content in plasma membranes and tonoplasts isolated from stored beet (Beta vulgaris L.) roots. The most significant differences between the membranes under all studied stresses were observed in the cholesterol content: it decreased in the plasmalemma, but increased 4-6 times in the tonoplast. Similar changes were observed for other sterols, but in different ways under different types of stress. Particularly noticeable differences were noted under hyperosmotic stress. The increase in the content of sterols was much more pronounced in the vacuolar membrane compared to the plasmalemma. This observation allows us to conclude that tonoplast plays a more significant role in protection of the plant cell from stress compared to plasmalemma.

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氧化和渗透胁迫下质膜和张力质体固醇含量变化的比较
我们比较了氧化和渗透胁迫诱导的甜菜根质膜和细胞体中甾醇含量的变化。在所有研究的压力下,细胞膜之间最显著的差异是胆固醇含量:在质膜中胆固醇含量下降,但在细胞膜中胆固醇含量增加了4-6倍。在其他固醇中也观察到类似的变化,但在不同类型的压力下变化的方式不同。高渗胁迫下的差异尤为明显。与质膜相比,液泡膜中固醇含量的增加更为明显。这一观察结果使我们得出结论,与质膜相比,质膜在保护植物细胞免受胁迫方面起着更重要的作用。
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
28
期刊介绍: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology   is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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