{"title":"Erythrocuprein, also Known as Superoxide Dismutase, Is a Hydroquinone Oxidase, and Imparts Resistance to Mitomycin C","authors":"P. Penketh","doi":"10.20455/ros.2022.c803","DOIUrl":null,"url":null,"abstract":"The enzymatic function of superoxide dismutase (SOD) is proposed to be as a ubiquinol oxidase. The SOD activity of this protein is likely a consequence of the necessary dismutation of superoxide (O2˙ˉ), generated as an enzyme-bound intermediate during its normal activity. The relatively low specificity of this enzyme for hydroquinones allowed it to oxidize a wide range of hydroquinone substrates. The general hydroquinone oxidase activity of this enzyme would thus enable it to behave as a mammalian analogue to bacterial mitomycin C resistance protein (MCRA). This would account for its elevated activity in cells expressing resistance against mitomycin C, porfiromycin, and related analogues, since superoxide itself is relatively nontoxic.","PeriodicalId":91793,"journal":{"name":"Reactive oxygen species (Apex, N.C.)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reactive oxygen species (Apex, N.C.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20455/ros.2022.c803","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The enzymatic function of superoxide dismutase (SOD) is proposed to be as a ubiquinol oxidase. The SOD activity of this protein is likely a consequence of the necessary dismutation of superoxide (O2˙ˉ), generated as an enzyme-bound intermediate during its normal activity. The relatively low specificity of this enzyme for hydroquinones allowed it to oxidize a wide range of hydroquinone substrates. The general hydroquinone oxidase activity of this enzyme would thus enable it to behave as a mammalian analogue to bacterial mitomycin C resistance protein (MCRA). This would account for its elevated activity in cells expressing resistance against mitomycin C, porfiromycin, and related analogues, since superoxide itself is relatively nontoxic.