The effect of natural deep eutectic solvents on laccase activity and oligomerization of rutin

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biocatalysis and Biotransformation Pub Date : 2022-09-08 DOI:10.1080/10242422.2022.2120391

A. Ünlü, B. Prasad, Kishan Anavekar, P. Bubenheim, A. Liese

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引用次数: 1

Abstract

Abstract Natural deep eutectic solvents (NADESs) were investigated for the activity and stability of the laccase enzyme. Laccase was found to be active only in a low amount of choline chloride containing NADESs while it maintained its activity both in high and low amounts of betaine containing NADESs. Rutin, as a flavonoid monomer shows high antioxidant, antibacterial, antiviral and anti-inflammatory properties and these properties are enhanced in the polymerised form. In this study, the organic co-solvent that is conventionally used in the oligomerization of rutin was replaced with two different green solvents, choline chloride-ethylene glycol (1:2 molar ratio) and betaine-mannose (5:2 molar ratio). LC-MS results revealed spontaneous derivatization of rutin as well as the oligomerization of the derivatives besides rutin. The final products were found to have enhanced superoxide radical activity. In this study, enzymatic oligomerization of rutin was investigated for the first time in the presence of eco-friendly green solvents presenting an alternative pathway without any toxic components.

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天然深共晶溶剂对漆酶活性和芦丁低聚反应的影响

摘要研究了天然深共晶溶剂(NADESs)对漆酶活性和稳定性的影响。漆酶仅在低含量的氯化胆碱中具有活性，而在高含量和低含量的甜菜碱中均保持活性。芦丁作为一种类黄酮单体，具有较高的抗氧化、抗菌、抗病毒和抗炎特性，这些特性在聚合形式下得到增强。在本研究中，用氯化胆碱-乙二醇(1:2摩尔比)和甜菜碱-甘露糖(5:2摩尔比)两种不同的绿色溶剂代替了常规用于芦丁低聚反应的有机共溶剂。液相色谱-质谱分析结果显示芦丁的自发衍生化反应以及除芦丁外的衍生物的寡聚反应。最终产物被发现具有增强的超氧自由基活性。在这项研究中，首次研究了在环保绿色溶剂存在下，芦丁的酶促低聚反应，提出了一种不含任何有毒成分的替代途径。

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来源期刊

Biocatalysis and Biotransformation 生物-生化与分子生物学

CiteScore

4.40

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： Biocatalysis and Biotransformation publishes high quality research on the application of biological catalysts for the synthesis, interconversion or degradation of chemical species. Papers are published in the areas of: Mechanistic principles Kinetics and thermodynamics of biocatalytic processes Chemical or genetic modification of biocatalysts Developments in biocatalyst''s immobilization Activity and stability of biocatalysts in non-aqueous and multi-phasic environments, including the design of large scale biocatalytic processes Biomimetic systems Environmental applications of biocatalysis Metabolic engineering Types of articles published are; full-length original research articles, reviews, short communications on the application of biotransformations, and preliminary reports of novel catalytic activities.