Soil Microbial Composition and Soil Health of Reverse-Osmosis-Concentrate and Brackish-Groundwater Irrigated Soils in Southern New Mexico

Abstract

The phospholipid fatty acid method was used to determine the shifts in microbial biomass due to irrigation with reverse-osmosis (RO) concentrate (or highly saline reject water) and brackish groundwater (BGW). In this greenhouse study, RO concentrate and BGW were applied to irrigate pecan trees for 8 months for two consecutive seasons. The objectives of the study were to (i) evaluate how irrigation with RO concentrate and BGW impacts soil microbial composition in pecan rhizospheres using microbial phospholipid fatty acid (PLFA) biomarkers as indicators, and (ii) evaluate its implications on soil health. Three treatments of RO concentrate (EC = 8.0 dS/m), BGW (EC = 4.0 dS/m), and the city of Las Cruces’s water (EC = 0.8 dS/m) as a control were used to irrigate pecan trees. EC, pH, and organic matter (OM%) content of the soil samples were measured, and PLFA biomarkers for the microbial community were determined. Na-, Cl-, and K-ion concentrations were 26.16, 32.54, and 5.93 meq/L in 2017 and 25.44, 24.26, and 5.49 meq/L in 2018, respectively, in RO irrigation pots. For two seasons, gram-positive bacteria were dominant, while gram-negative bacteria were not detected in the second season. PLFA biomarkers of fungi were found among all three treatments in the first season; however, they appeared only with BGW in the second season. Actinomycetes were recorded in the first season while they were not seen in the second season. Increases in soil salinity and microbial shifts could have important implications for soil health. Irrigating with RO and BGW shifted the soil microbial composition; therefore, long-term irrigation with BGW and RO concentrate would be deleterious for pecan production and soil health.