Mechanism of Placenta Damage in Gestational Diabetes Mellitus by Investigating TXNIP of Patient Samples and Gene Functional Research in Cell Line.

IF 2.8 3区医学 Q3 ENDOCRINOLOGY & METABOLISM Diabetes Therapy Pub Date : 2019-12-01 Epub Date: 2019-10-26 DOI:10.1007/s13300-019-00713-z

Sarina, Dong Fang Li, Zong Qi Feng, Jie Du, Wen Hua Zhao, Na Huang, Jian Chao Jia, Zhou Ying Wu, Alamusi, Yong Yun Wang, Xiao Li Ji, Lan Yu

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Abstract

Introduction: Gestational diabetes mellitus (GDM) is a gestational complication that affects maternal and child health. The placenta provides the fetus with the necessary nutrition and oxygen and takes away the metabolic waste. Patients with GDM are diagnosed and treated merely on the basis of the blood glucose level; this approach does nothing to help evaluate the status of the placenta, which is worth noting in GDM. The purpose of this research was to clarify the relation between thioredoxin-interacting protein (TXNIP) and reactive oxygen species (ROS) in the placenta of patients with GDM, which has thus far remained unclear.

Methods: The expression of TXNIP in the placentas of 10 patients with GDM and 10 healthy puerperae (control group) was investigated via immunofluorescence. The relation among TXNIP, ROS, and the function of mitochondria was explored in HTR-8/SVneo cells stimulated by high glucose (HG).

Results: The results showed the expression of TXNIP in the placentas of patients with GDM was higher than that in the control group, and the expression of TXNIP in HTR-8/SVneo cells treated with HG was higher than that in the control group, causing the accumulation of ROS and changes of mitochondria, promoting apoptosis and inhibition of migration.

Conclusions: High expression of TXNIP caused by HG mediates the increasing ROS and the mitochondria dysfunction in GDM; this impairs the function of the placenta and is the basis for the prediction of perinatal outcome.

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妊娠期糖尿病患者血清TXNIP及细胞系基因功能研究胎盘损伤机制

简介妊娠糖尿病（GDM）是一种影响母婴健康的妊娠并发症。胎盘为胎儿提供必要的营养和氧气，并带走代谢废物。GDM 患者的诊断和治疗仅以血糖水平为依据，这种方法无助于评估胎盘的状态，而胎盘的状态在 GDM 中值得注意。本研究的目的是阐明 GDM 患者胎盘中硫氧还蛋白相互作用蛋白（TXNIP）与活性氧（ROS）之间的关系：方法：通过免疫荧光法研究了 TXNIP 在 10 例 GDM 患者和 10 例健康孕妇（对照组）胎盘中的表达。方法：通过免疫荧光法研究了 10 名 GDM 患者和 10 名健康产儿（对照组）胎盘中 TXNIP 的表达，并在高糖（HG）刺激下的 HTR-8/SVneo 细胞中探讨了 TXNIP、ROS 和线粒体功能之间的关系：结果表明：GDM 患者胎盘中 TXNIP 的表达高于对照组，HG 处理的 HTR-8/SVneo 细胞中 TXNIP 的表达高于对照组，导致 ROS 的积累和线粒体的变化，促进细胞凋亡并抑制迁移：结论：HG引起的TXNIP高表达介导了ROS的增加和GDM的线粒体功能障碍；这损害了胎盘的功能，是预测围产期结局的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diabetes Therapy Medicine-Endocrinology, Diabetes and Metabolism

自引率

7.90%

发文量

130

期刊介绍： Diabetes Therapy is an international, peer reviewed, rapid-publication (peer review in 2 weeks, published 3–4 weeks from acceptance) journal dedicated to the publication of high-quality clinical (all phases), observational, real-world, and health outcomes research around the discovery, development, and use of therapeutics and interventions (including devices) across all areas of diabetes. Studies relating to diagnostics and diagnosis, pharmacoeconomics, public health, epidemiology, quality of life, and patient care, management, and education are also encouraged. The journal is of interest to a broad audience of healthcare professionals and publishes original research, reviews, communications and letters. The journal is read by a global audience and receives submissions from all over the world. Diabetes Therapy will consider all scientifically sound research be it positive, confirmatory or negative data. Submissions are welcomed whether they relate to an international and/or a country-specific audience, something that is crucially important when researchers are trying to target more specific patient populations. This inclusive approach allows the journal to assist in the dissemination of all scientifically and ethically sound research.