Effects of probiotics on apoptosis and proliferation of intestinal epithelial cells in rats with necrotizing enterocolitis

Q4 Medicine 中华实用儿科临床杂志 Pub Date : 2020-03-19 DOI:10.3760/CMA.J.CN101070-20190125-00059

Mengmeng Yuan, Xiao-yun Zhang, Yaping Song, Mingchao Li

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Abstract

Objective To study the effect of compound Lactobacillus acidophilus on the apoptosis of intestinal epithelial cells and expression of proliferating cell nuclear antigen (PCNA) in rats with necrotizing enterocolitis, so as to provide some theoretical reference for the application of compound Lactobacillus acidophilus in the prevention and treatment of necrotizing enterocolitis(NEC) for newborn infants. Methods Thirty-six 48-hour old newborn SD rats were randomly divided into normal group, model group and intervention group, with 12 rats in each group.The NEC model was established by hypoxia and cold stimulation for 3 days, and the intervention group was given the compound Lactobacillus acidophilus at the same time; after the model was established, all the rats were sacrificed after 12 hours of fasting and their intestinal tissues were excised by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) method to determine the condition of apoptosis and to detect expression of the PCNA gene in the intestinal tissues by fluorescent quantitative PCR. Results (1) The apoptosis rates of the intestinal tissues: in the model group, the normal group and the intervention group were (88.33±2.77)%, (4.75±0.75)% and (44.41±4.81)%, respectively, and the differences had statistical significance(all P<0.05). (2) The PCNA expression in the intestinal tissue by real-time PCR in the model group, normal group and intervention group were (9.28±3.26)×10-4, (15.35±1.91)×10-4 and (12.09±3.06)×10-4, respectively, and the differences had statistical significance(all P<0.05). Conclusions The apoptosis rate of intestinal epithelial cells increases and PCNA expression decreases when NEC occurs.Compound Lactobacillus acidophilus can reduce apoptosis, promote intestinal epithelial cell proliferation, and thus help to reduce the risk and severity of NEC in neonatal rats. Key words: Neonatal necrotizing enterocolitis; Apoptosis; Proliferating cell nuclear antigen; Compound Lactobacillus acidophilus

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益生菌对坏死性小肠结肠炎大鼠肠上皮细胞凋亡和增殖的影响

目的研究复方嗜酸乳杆菌对坏死性小肠结肠炎大鼠肠上皮细胞凋亡及增殖细胞核抗原（PCNA）表达的影响，为复方嗜酸乳杆菌在新生儿坏死性小肠结肠炎防治中的应用提供一定的理论参考。方法36只48小时新生SD大鼠随机分为正常组、模型组和干预组，每组12只。通过缺氧和冷刺激建立NEC模型3天，干预组同时给予复方嗜酸乳杆菌；模型建立后，禁食12小时处死所有大鼠，用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）切除肠组织，以确定细胞凋亡情况，并用荧光定量PCR检测肠组织中PCNA基因的表达。结果（1）肠组织凋亡率：模型组、正常组和干预组分别为（88.33±2.77）%、（4.75±0.75）%和（44.41±4.81）%，差异有统计学意义（均P<0.05），×10-4和（12.09±3.06）×10-4，差异有统计学意义（均P<0.05）。复方嗜酸乳杆菌可以减少细胞凋亡，促进肠上皮细胞增殖，从而有助于降低新生大鼠NEC的风险和严重程度。关键词：新生儿坏死性小肠结肠炎；细胞凋亡；增殖细胞核抗原；复方嗜酸乳杆菌

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来源期刊

中华实用儿科临床杂志 Medicine-Pediatrics, Perinatology and Child Health

CiteScore

0.60

自引率

0.00%

发文量

14243

期刊介绍： Chinese Journal of Applied Clinical Pediatrics ( semi-monthly ) is a core journal of paediatrics under the supervision of China Association for Science and Technology, sponsored by Chinese Medical Association and undertaken by Xinxiang Medical College. Founded in 1986, it is openly circulated both at home and abroad. The journal has several columns, such as Expert Forum, Experimental Research and Paediatric Surgery, which are mainly for paediatric medical workers and medical researchers in hospitals. Its purpose is to reflect the new theories and technologies in paediatric medicine and scientific research at home and abroad, and to promote academic exchanges. Chinese Journal of Applied Clinical Pediatrics is a source journal of China Science Citation Database (CSCD), a core journal of Peking University, a source journal of Chinese science and technology paper statistics (China Science and Technology Core Journals), a core academic journal of RCCSE, a high-quality scientific and technical journal of China, a high-quality scientific and technical journal of China Association for Science and Technology, and a high-quality scientific and technical journal of China Biomedical Science and Technology Association. We have been published in China Biomedical Literature Database (SinoMed), China Knowledge Network, Wanfang Data Knowledge Service Platform, China Academic Journal Abstracts, Scopus Database, Chemical Abstracts (USA), Japan Science and Technology Agency (JSTA) Database, Copernicus Abstracts (Poland), Abstracts of the Centre for Agricultural and Biological Sciences (CABS) of the United Kingdom, Cambridge Scientific Abstracts ProQuest Database, WHO Medical Journal of the Western Pacific Region (WMPR), and WHO Medical Journal of the Western Pacific Region (WMPR) of the United States. We have been included in dozens of authoritative databases at home and abroad, such as WHO Western Pacific Region Index of Medicine (WPRIM), Ullrich's Guide to Periodicals, and so on.