In Vitro Anti-Cancer Activity of Larval Hemolymph and Fat Body of Flesh Fly Sarcophaga argyrostoma (Diptera: Sarcophagidae)

S. Mahmoud, L. El-Khashab, W. Moselhy, A. Zayed, M. Salama
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引用次数: 1

Abstract

Insects are well recognized as a source of potentially useful compounds for modern medicine. Development of anticancer drugs from natural resources has been performed throughout the world. In the present study, anticancer activity of the hemolymph and fat body of Sarcophaga argyrostoma third larval instars is assayed against human breast cancer cell line (MDA-MB-231 cells). The cytotoxicity of the hemolymph and fat body samples were determined. The results showed that growth of MDA-MB-231 cells was inhibited at different concentrations upon 24 h of exposure. There is no inhibitory activity against Vero cells under these experimental conditions. Protein profile of the hemolymph and fat body were extracted and separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Protein analysis showed differences in number of electrophoretic protein bands with different molecular weights between treated and control larvae. The present work assumes that hemolymph and fat body tissue have cytotoxicity against MDA-MB-231 cells and these results exhibit that larvae from S. argyrostoma could be used as a good source for developing anti-cancer agents and knowledge of these anti-cancer compounds will lead to better control of human disease.
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银口蝇幼虫血淋巴和脂肪体的体外抗癌活性
昆虫被公认为现代医学中潜在有用化合物的来源。利用自然资源开发抗癌药物已经在世界各地进行。在本研究中,测定了三龄石首鱼血淋巴和脂肪体对人乳腺癌症细胞系(MDA-MB-231细胞)的抗癌活性。测定了血淋巴和脂肪体样品的细胞毒性。结果表明,不同浓度的MDA-MB-231细胞在暴露24小时后生长受到抑制。在这些实验条件下对Vero细胞没有抑制活性。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)提取并分离血淋巴和脂肪体的蛋白质图谱。蛋白质分析显示,处理和对照幼虫之间具有不同分子量的电泳蛋白带的数量存在差异。目前的工作假设血淋巴和脂肪体组织对MDA-MB-231细胞具有细胞毒性,并且这些结果表明,来自银杉的幼虫可以用作开发抗癌剂的良好来源,并且对这些抗癌化合物的了解将导致更好地控制人类疾病。
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