In Vitro Anti-Cancer Activity of Larval Hemolymph and Fat Body of Flesh Fly Sarcophaga argyrostoma (Diptera: Sarcophagidae)

S. Mahmoud, L. El-Khashab, W. Moselhy, A. Zayed, M. Salama
{"title":"In Vitro Anti-Cancer Activity of Larval Hemolymph and Fat Body of Flesh Fly Sarcophaga argyrostoma (Diptera: Sarcophagidae)","authors":"S. Mahmoud, L. El-Khashab, W. Moselhy, A. Zayed, M. Salama","doi":"10.4236/ae.2020.82007","DOIUrl":null,"url":null,"abstract":"Insects are well recognized as a source of potentially useful compounds for modern medicine. Development of anticancer drugs from natural resources has been performed throughout the world. In the present study, anticancer activity of the hemolymph and fat body of Sarcophaga argyrostoma third larval instars is assayed against human breast cancer cell line (MDA-MB-231 cells). The cytotoxicity of the hemolymph and fat body samples were determined. The results showed that growth of MDA-MB-231 cells was inhibited at different concentrations upon 24 h of exposure. There is no inhibitory activity against Vero cells under these experimental conditions. Protein profile of the hemolymph and fat body were extracted and separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Protein analysis showed differences in number of electrophoretic protein bands with different molecular weights between treated and control larvae. The present work assumes that hemolymph and fat body tissue have cytotoxicity against MDA-MB-231 cells and these results exhibit that larvae from S. argyrostoma could be used as a good source for developing anti-cancer agents and knowledge of these anti-cancer compounds will lead to better control of human disease.","PeriodicalId":58873,"journal":{"name":"昆虫学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"昆虫学(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/ae.2020.82007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

Insects are well recognized as a source of potentially useful compounds for modern medicine. Development of anticancer drugs from natural resources has been performed throughout the world. In the present study, anticancer activity of the hemolymph and fat body of Sarcophaga argyrostoma third larval instars is assayed against human breast cancer cell line (MDA-MB-231 cells). The cytotoxicity of the hemolymph and fat body samples were determined. The results showed that growth of MDA-MB-231 cells was inhibited at different concentrations upon 24 h of exposure. There is no inhibitory activity against Vero cells under these experimental conditions. Protein profile of the hemolymph and fat body were extracted and separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Protein analysis showed differences in number of electrophoretic protein bands with different molecular weights between treated and control larvae. The present work assumes that hemolymph and fat body tissue have cytotoxicity against MDA-MB-231 cells and these results exhibit that larvae from S. argyrostoma could be used as a good source for developing anti-cancer agents and knowledge of these anti-cancer compounds will lead to better control of human disease.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
银口蝇幼虫血淋巴和脂肪体的体外抗癌活性
昆虫被公认为现代医学中潜在有用化合物的来源。利用自然资源开发抗癌药物已经在世界各地进行。在本研究中,测定了三龄石首鱼血淋巴和脂肪体对人乳腺癌症细胞系(MDA-MB-231细胞)的抗癌活性。测定了血淋巴和脂肪体样品的细胞毒性。结果表明,不同浓度的MDA-MB-231细胞在暴露24小时后生长受到抑制。在这些实验条件下对Vero细胞没有抑制活性。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)提取并分离血淋巴和脂肪体的蛋白质图谱。蛋白质分析显示,处理和对照幼虫之间具有不同分子量的电泳蛋白带的数量存在差异。目前的工作假设血淋巴和脂肪体组织对MDA-MB-231细胞具有细胞毒性,并且这些结果表明,来自银杉的幼虫可以用作开发抗癌剂的良好来源,并且对这些抗癌化合物的了解将导致更好地控制人类疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
89
期刊最新文献
Preliminary Study of Predation Effectiveness of the Assassin Bug Rhynocoris rapax Stal (Heteroptra: Reduviidae) on Fall Armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae), a Major Pest of Maize Crops Inventory and Biodiversity of the Entomofauna Associated with Shea Fruits (Vitellaria paradoxa C. F. Gaertn), an Important Non-Timber Forest Product in the Ziro Province of Burkina Faso Chemical Composition of the Neochetina eichhorniae and Neochetina bruchi and Their Potential for Use as Livestock Feed Chemical Composition and Use of Momordica charantia L. and Hyptis spicigera Lam. Extracts as Mosquito Larvicides and Insect Growth Regulators against Malarial Vector, Anopheles gambiae Giles Chemical Composition, Larvicidal and Adult Emergence Inhibition Activities of Balanites aegyptiaca Del. Seed and Aristolochia albida Duch. Root Extracts against Malaria Vector, Anopheles gambiae Giles
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1