Dysbiotic changes of periodontal pathogens in patients wearing conventional and self-ligating orthodontic appliances

Abstract

Abstract Objective: This study aimed to analyse dysbiotic changes of periodontal pathogenic bacteria and their relationship with different types of fixed orthodontic appliances in a population located in Northwestern Mexico. Methods: Three groups of patients were identified: a control group without orthodontic appliances (C), a conventional-ligating appliance group (CLA), and a self-ligating appliance group (SLA). Periodontal biofilm samples were collected for DNA extraction to identify the presence and load of Treponema denticola, Prevotella intermedia, and Fusobacterium nucleatum, using a quantitative real-time PCR technique. Results: A total of 92 patients were included. The results showed that F. nucleatum was present in all groups including the control patients (C 96%, CLA 100%, and SLA 67%, respectively). Female participants displayed a higher frequency of periodontal pathogens than males, but males were more affected by F. nucleatum. In addition, the presence of T. denticola and P. intermedia was time-dependent, being more frequent in patients in treatment for longer than 12 months whereas CLA showed 74% and 78% of positive samples and SLA showed 78% and 89%, respectively. F. nucleatum was present in 100% of CLA samples before and after 12 months of treatment and its load was higher in the SLA group after 12 months. Conclusions: Dysbiotic changes that could affect the periodontal tissues were seen in patients wearing orthodontic appliances. The frequency of F. nucleatum was significantly higher in CLA and noted with a greater load in SLA. In addition, female participants showed a higher frequency of periodontal pathogens while male subjects were more affected by F. nucleatum. As expected, treatment for longer than 12 months correlated with a higher frequency of all periodontal pathogens. The results support the concept that dysbiosis leading to periodontal disease can be caused by the rise of a dominant species, instead of the appearance of a new species.