Organization of histo-hematic barriers of the liver in Anglo-Nubian goat

Abstract

The aim of this research was to establish features of the liver histo-hematic barriers ultrastructural organization of the Anglo-Nubian goat. The liver of an adult Anglo-Nubian goat was used as the material. The work was carried out using the electron microscopic method. Liver parenchymal tissue fragments were selected. These samples were fixed in a 2.0% glutaraldehyde solution on a cacodylate buffer for two hours. They were then washed in three portions of the same buffer and post-fixed in a 1.0% solution of osmium tetrachloride for one hour. The samples were then dehydrated in alcohols of ascending concentration and absolute acetone. The subsequent filling of the fragments was carried out in Epon-812. Ultrathin sections were obtained on an ultramicrotome, contrasted with a 2.0% aqueous solution of uranyl acetate and a solution of lead citrate. The ultrathin sections were photographed with a Jem-1011 electron microscope at magnifications of 2500-3000. Two histo-hematic barriers are detected in the liver of the studied animals hemato-hepatic and hepatobiliary. The hemato-hepatic barrier is formed by the plasmalemma of the apical end of the hepatocyte, covered by the glycocalyx, the perisinusoidal space of the Disse, the endotheliocyte of the sinusoid capillary, as well as Kupfer cells located in the lumen of the latter. The hepatobiliary includes all of the above structures, with the exception of Kupfer cells, as well as the plasmalemma of the basal end of the hepatocyte. All of the above structures in their organization have characteristic species features for Anglo-Nubian goats.