Molecular Markers (RAPD and SSR) Based Characterisation of Genetic Diversity and Population Structure of Moss Hyophila Involuta

Abstract

Genetic relationships among 24 genotypes of Hyophila involuta collected from five different natural populations of Mount Abu (Rajasthan) is analysed using RAPD and SSR markers. Based on efficiency parameters calculated for each marker system such as polymorphic information content (RAPD = 0.34; SSR = 0.66), marker index (RAPD = 2.78; SSR = 2.62) and resolving power (RAPD = 8.13; SSR = 2.23), the RAPD marker system shows higher values for some indices but microsatellites are more accurately reproducible than RAPD. Moreover, in case of the SSR, the average number of alleles was almost twice compared to RAPD. Mean coefficient of genetic differentiation between populations with RAPD was Gst = 0.269, while with SSR marker was Fst = 0.224. The UPGMA cluster analysis assembled genotypes into two main clusters with diverse levels of sub-clustering within the clusters. Also, the Mantel test showed no significant correlation between geographical and genetic distances. The observed moderately high genetic variability can be explained by efficient spore dispersal. Other factors such as reproductive mode, somatic mutation, continuous propagule recruitment and high degree of intermingling have great impact on the level of genetic variability in moss populations.