POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE

Revista Brasileira de Milho e Sorgo Pub Date : 2020-11-16 DOI:10.18512/rbms2020v19e1190

E. Gomes, U.G. Paula Lana, Marina Eleutério Gói De Moraes Rodrigues, S. M. de Sousa, C. Oliveira, Amanda Nayê GUIMARÃES TAVARES

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引用次数: 3

Abstract

The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subitilis (B2082 and B2084) using 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants production quality control, strains traceability and to detail PGPB colonization.

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玉米芽孢杆菌菌株多相特性研究

使用具有植物生长促进细菌（PGPB）的生物接种剂是一种可持续的农业实践，在世界范围内不断扩大。因此，对这些细菌进行分子表征是为生物接种剂生产提供更好的安全性和可追溯性的基础。本工作旨在鉴定、表征和开发与植物生长促进有关的芽孢杆菌菌株的特异性分子标记。利用16S rRNA基因测序和MALDI-TOF质谱法鉴定出5株菌株，分别为短小芽孢杆菌（B32）、苏云金芽孢杆菌（B116）、巨大芽孢杆菌（B119）和亚tilis芽孢杆菌（B2082和B2084）。重复元件回文（Rep-PCR）和扩增核糖体DNA限制性分析（ARDRA）技术允许对菌株进行区分，但B2082和B2084表现出相同的遗传图谱，表明它们是相同或遗传上接近的分离株。此外，针对B116和B119菌株开发了特异性分子标记，可用于微生物接种物生产质量控制、菌株可追溯性和详细的PGPB定殖。

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Revista Brasileira de Milho e Sorgo

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40 weeks