Improving the quality of rooster semen frozen in straws by screening the glycerol concentration and freezing rate

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE British Poultry Science Pub Date : 2020-03-03 DOI:10.1080/00071668.2019.1686126

B. Wu, X. Yang, H. Yan

{"title":"Improving the quality of rooster semen frozen in straws by screening the glycerol concentration and freezing rate","authors":"B. Wu, X. Yang, H. Yan","doi":"10.1080/00071668.2019.1686126","DOIUrl":null,"url":null,"abstract":"ABSTRACT 1. This study examined different glycerol concentrations (GC) and freezing rates to improve the quality of rooster spermatozoa frozen in straws, and to determine the effect of varying GC on post-thawed spermatozoa quality, as evaluated by fertility and hatchability. 2.The experiment included two tests. In test 1, rooster semen straws containing 2, 4, 6, 8 and 11% glycerol were put in a rack (nine tiers with a 1 cm interval between every two tiers, 1 to 9 cm above liquid nitrogen (LN) source), and gradually frozen. The semen straws located in different tiers experienced different temperatures and freezing rates. The straws were then thawed and live sperm numbers determined. In test 2, rooster semen straws containing 2, 4, 6, 8 and 11% glycerol were put on optimal tiers (identified in test 1) for freezing, and stored at −196°C. Hens were inseminated with the frozen semen (post-thawed and glycerol removed, about 4.0 × 108 sperm per hen), and eggs incubated. 3. The numbers of live sperm in the 11% glycerol group was higher than that in 2, 4 or 6% glycerol group (P < 0.05) for the semen straws on tiers 1 to 9, while that on tiers 1 to 5 was lower than that on tier 6 to 8 (P < 0.05). GC, freezing rate and the interaction between GC and freezing rate had a significant effect on live sperm numbers (P < 0.01). The highest fertility was in the 6% glycerol group and occurred on day 5 after insemination. The lowest fertility occurred in the 2% glycerol group on day 10 after insemination. 4. The optimal combination was 11% glycerol in straws located 6 cm above the LN surface (on tier 6). The 6% glycerol group achieved the highest fertility (77.6%), which surpassed that reported in recent years.","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":"61 1","pages":"173 - 179"},"PeriodicalIF":1.7000,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00071668.2019.1686126","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2019.1686126","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 3

Abstract

ABSTRACT 1. This study examined different glycerol concentrations (GC) and freezing rates to improve the quality of rooster spermatozoa frozen in straws, and to determine the effect of varying GC on post-thawed spermatozoa quality, as evaluated by fertility and hatchability. 2.The experiment included two tests. In test 1, rooster semen straws containing 2, 4, 6, 8 and 11% glycerol were put in a rack (nine tiers with a 1 cm interval between every two tiers, 1 to 9 cm above liquid nitrogen (LN) source), and gradually frozen. The semen straws located in different tiers experienced different temperatures and freezing rates. The straws were then thawed and live sperm numbers determined. In test 2, rooster semen straws containing 2, 4, 6, 8 and 11% glycerol were put on optimal tiers (identified in test 1) for freezing, and stored at −196°C. Hens were inseminated with the frozen semen (post-thawed and glycerol removed, about 4.0 × 108 sperm per hen), and eggs incubated. 3. The numbers of live sperm in the 11% glycerol group was higher than that in 2, 4 or 6% glycerol group (P < 0.05) for the semen straws on tiers 1 to 9, while that on tiers 1 to 5 was lower than that on tier 6 to 8 (P < 0.05). GC, freezing rate and the interaction between GC and freezing rate had a significant effect on live sperm numbers (P < 0.01). The highest fertility was in the 6% glycerol group and occurred on day 5 after insemination. The lowest fertility occurred in the 2% glycerol group on day 10 after insemination. 4. The optimal combination was 11% glycerol in straws located 6 cm above the LN surface (on tier 6). The 6% glycerol group achieved the highest fertility (77.6%), which surpassed that reported in recent years.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

通过甘油浓度和冷冻速率的筛选提高稻草冷冻公鸡精液的质量

抽象的1。本研究研究了不同的甘油浓度(GC)和冷冻速率，以提高公鸡精子在稻草中冷冻的质量，并确定了不同的GC对解冻后精子质量的影响，通过受精和孵化率来评估。2.实验包括两个测试。试验1将含2、4、6、8和11%甘油的公鸡精液吸管置于架上(9层，每层间隔1cm，液氮源上方1 ~ 9cm)，逐渐冷冻。位于不同层位的精液吸管经历了不同的温度和冷冻速率。然后将吸管解冻，并测定活精子的数量。在试验2中，将含有2,4,6,8和11%甘油的公鸡精液吸管放入最佳层(在试验1中确定)进行冷冻，并在- 196°C保存。用冷冻精液(解冻后去除甘油，每只鸡约4.0 × 108个精子)进行受精，孵卵。3.第1 ~ 9层精液吸管的活精子数，11%甘油组高于2、4和6%甘油组(P < 0.05)，第1 ~ 5层低于第6 ~ 8层(P < 0.05)。GC、冷冻速率以及GC与冷冻速率的交互作用对活精子数有极显著影响(P < 0.01)。6%甘油组受精率最高，发生在受精后第5天。2%甘油组受精率最低，发生在授精后第10天。4. 在距LN表面6 cm处(第6层)的秸秆中添加11%甘油是最佳组合。6%甘油组的肥力最高(77.6%)，超过了近年报道的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .