Influence of terms and methods of storage on changes in enzyme activity of meadow chernozem-like soil on Zeya-Bureya plain

Q4 Agricultural and Biological Sciences Biulleten'' Pochvennogo instituta im VV Dokuchaeva Pub Date : 2022-12-10 DOI:10.19047/0136-1694-2022-112-48-72
O. Piletskaya, D. A. Yachnaya
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Abstract

The analysis of the biological and enzymatic properties of soils is an important aspect of soil ecology, but the results of studies can be strongly influenced by the storage conditions of the samples. Variation in storage methods and duration studies reduces the ability to accurately interpret data and compare results. This work presents a study of the influence of various storage conditions and time on the results of the activity of enzymes of hydrolase class (urease, phosphatase), and oxidoreductases class (catalase, peroxidase and polyphenoloxidase) of meadow chernozem-like soil of the Zeya-Bureya plain. For the research, a laboratory experiment was performed, in which naturally moist and air-dry samples were taken and stored under various conditions: room temperature (+23 – +25 °С), low positive temperature (refrigerator, +10 °C), negative temperature (freezer, –10 °С) within 7, 28, 90 and 365 days. As a result, we found that drying soil samples immediately after sampling increases the activity of urease, phosphatase, peroxidase and polyphenoloxidase. Storage conditions do not significantly affect the activity of enzymes. To assess the actual enzymatic activity, we recommend using naturally moist soil samples immediatel y after sampling; to assess potential enzymatic activity, drying the soil and storing it for no more than 7 days are recommended. The results of this study provide useful information on the impact of sample storage conditions for researchers of enzyme activity in similar climates and contribute to further consideration and discussion of the implications of sample storage.
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贮藏条件和方法对泽亚-布雷亚平原草甸黑钙土酶活性变化的影响
土壤的生物学和酶学性质分析是土壤生态学的一个重要方面,但研究结果可能受到样品储存条件的强烈影响。存储方法和持续时间研究的差异降低了准确解释数据和比较结果的能力。本文研究了不同贮藏条件和贮存时间对泽亚布里亚平原黑土样草甸土壤水解酶类酶(脲酶、磷酸酶)和氧化还原酶类酶(过氧化氢酶、过氧化物酶和多酚氧化酶)活性的影响。为了进行研究,我们进行了一项实验室实验,在不同的条件下,在7、28、90和365天内,将自然潮湿和风干的样品保存在室温(+23 - +25°С)、低正温(冰箱,+10°C)、负温(冰箱,- 10°С)下。因此,我们发现在取样后立即干燥土壤样品会增加脲酶、磷酸酶、过氧化物酶和多酚氧化酶的活性。储存条件对酶的活性没有显著影响。为了评估实际的酶活性,我们建议在取样后立即使用自然潮湿的土壤样品;为了评估潜在的酶活性,建议将土壤干燥并存放不超过7天。本研究的结果为研究类似气候条件下酶活性的研究人员提供了有关样品储存条件影响的有用信息,并有助于进一步考虑和讨论样品储存的意义。
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
15
审稿时长
8 weeks
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