An experimental study to evaluate pathogenicity of Yersinia ruckeri isolated from rainbow trout (Oncorhynchus mykiss) in Caspian trout (Salmo caspius)

IF 0.8 4区农林科学 Q3 FISHERIES Iranian Journal of Fisheries Sciences Pub Date : 2020-09-10 DOI:10.22092/IJFS.2020.122408

M. Adel, M. Ina-Salwany, S. Yeganeh, I. Karimi, A. Zafariian

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引用次数: 1

Abstract

The main objective of this study was to evaluate the pathogenicity of isolated Yersinia ruckeri from Caspian trout. Y. ruckeri specimens were isolated from infected rainbow trout farms located in north of Iran. The identification was confirmed by biochemical tests and 16s rRNA gene sequencing. The pathogenicity test was carried out to determine the virulence of the Y. ruckeri by IP injection, and histopathological and hemato-biochemical changes were evaluated pre and post challenge. Based on the results, LD50 were calculated as 1×105 CFU/mL, while 1×107 CFU/mL caused 100% mortality after 10 days in the experimental groups. The main histopathological changes were seen in liver, kidney, spleen and intestine, including erosion and necrosis, infiltration of inflammatory cell, hyperplasia and catarrhal enteritis in the infected organs. Also, a significant decrease in glucose, RBC counts, Hb values and HCT percentage and significant increase in the WBC counts, neutrophils percentage, AST, ALP and LDH values were observed in infected fish after challenging. Finally, Caspian trout is susceptible to Yersiniosis and can play important role in transmission of Y. ruckeri to cultured and wild fish.

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虹鳟鱼分离的拉克氏耶尔森菌对里鳟鱼致病性的实验研究

本研究的主要目的是评价从里海鳟鱼中分离的鲁氏耶尔森菌的致病性。从伊朗北部受感染的虹鳟养殖场分离到了鲁克里氏菌标本。通过生物化学测试和16s rRNA基因测序证实了该鉴定。通过IP注射进行致病性试验以确定鲁氏菌的毒力，并评估攻击前后的组织病理学和血液生化变化。根据结果，LD50计算为1×105CFU/mL，而在实验组中，1×107CFU/mL在10天后导致100%的死亡率。肝、肾、脾和肠的组织病理学变化主要表现为感染器官的侵蚀坏死、炎性细胞浸润、增生和卡他性肠炎。此外，在攻击后，在受感染的鱼中观察到葡萄糖、RBC计数、Hb值和HCT百分比显著降低，WBC计数、中性粒细胞百分比、AST、ALP和LDH值显著增加。最后，里海鳟鱼易感染耶尔森菌病，并在将鲁氏菌传播给养殖和野生鱼类中发挥重要作用。

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.