Circulating microRNAs as biomarkers and mediators of platelet activation

IF 2.5 3区医学 Q3 CELL BIOLOGY Platelets Pub Date : 2022-03-09 DOI:10.1080/09537104.2022.2042236

C. Gutmann, M. Mayr

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引用次数: 5

Abstract

Abstract Platelets are essential mediators of physiological hemostasis and pathological thrombosis. Currently available tests and markers of platelet activation did not prove successful in guiding treatment decisions for patients with cardiovascular disease, justifying further research into novel markers of platelet reactivity. Platelets contain a variety of microRNAs (miRNAs) and are a major contributor to the extracellular circulating miRNA pool. Levels of platelet-derived miRNAs in the circulation have been associated with different measures of platelet activation as well as antiplatelet therapy and have therefore been implied as potential new markers of platelet reactivity. In contrast to the ex vivo assessment of platelet reactivity by current platelet function tests, miRNA measurements may enable assessment of platelet reactivity in vivo. It remains to be seen however, whether miRNAs may aid clinical diagnostics. Major limitations in the platelet miRNA research field remain the susceptibility to preanalytical variation, non-standardized sample preparation and data normalization that hampers inter-study comparisons. In this review, we provide an overview of the literature on circulating miRNAs as biomarkers of platelet activation, highlighting the underlying biology, the application in patients with cardiovascular disease and antiplatelet therapy and elaborating on technical limitations regarding their quantification in the circulation.

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循环microrna作为血小板活化的生物标志物和介质

血小板是生理性止血和病理性血栓形成的重要介质。目前可用的血小板活化测试和标志物在指导心血管疾病患者的治疗决策方面并不成功，这证明进一步研究血小板反应性的新标志物是合理的。血小板含有多种microrna (miRNA)，是细胞外循环miRNA库的主要贡献者。循环中血小板来源的mirna水平与血小板活化的不同措施以及抗血小板治疗有关，因此被认为是血小板反应性的潜在新标志物。与目前通过血小板功能测试对血小板反应性的体外评估不同，miRNA测量可以在体内评估血小板反应性。然而，mirna是否有助于临床诊断还有待观察。血小板miRNA研究领域的主要局限性仍然是易受分析前变异、非标准化样品制备和数据归一化的影响，这妨碍了研究间的比较。在这篇综述中，我们提供了关于循环mirna作为血小板活化的生物标志物的文献综述，重点介绍了潜在的生物学，在心血管疾病患者和抗血小板治疗中的应用，并详细说明了在循环中量化mirna的技术局限性。

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来源期刊

Platelets 医学-细胞生物学

CiteScore

6.70

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： Platelets is an international, peer-reviewed journal covering all aspects of platelet- and megakaryocyte-related research. Platelets provides the opportunity for contributors and readers across scientific disciplines to engage with new information about blood platelets. The journal’s Methods section aims to improve standardization between laboratories and to help researchers replicate difficult methods. Research areas include: Platelet function Biochemistry Signal transduction Pharmacology and therapeutics Interaction with other cells in the blood vessel wall The contribution of platelets and platelet-derived products to health and disease The journal publishes original articles, fast-track articles, review articles, systematic reviews, methods papers, short communications, case reports, opinion articles, commentaries, gene of the issue, and letters to the editor. Platelets operates a single-blind peer review policy. Authors can choose to publish gold open access in this journal.