Anti-Inflammatory and Neuroprotective Effect of the Anti-Obesity Dietary Supplement Dekosilhue® in an In Vitro Model of Neuroinflammation

Abstract

Obesity is now recognized as a global health problem and has reached epidemic proportions, affecting both developed and developing countries. This condition is associated with an increased incidence in central nervous system (CNS) disorders. The intake of foods or supplements with anti-inflammatory activity could potentially lead to a lower impact of obesity on the functionality of CNS. Therefore, in this work we tested the effect of a dietary supplement, DEKOSILHUE® (DSK), containing polyphenols enriched herbal extracts and successfully used for improving the control of body weight, in an in vitro inflammatory model. LPS-stimulated immortalized microglia BV2 cells have been used as a model of neuroinflammation. The stimulation with LPS increased the release and activation of several neuroinflammatory mediators, such as IKB⍺, HDAC-1, and IL-1ß. DSK reduced the levels and the activation of all these pro-inflammatory markers. In addition, DSK increased the expression of the anti-inflammatory cytokine IL-10, promoting an anti-inflammatory response. Finally, we showed that the anti-inflammatory effect of DSK can prevent the neurotoxicity related to microglia hyperactivation. In conclusion, DSK could potentially help in controlling neuroinflammation CNS, reducing microglia pro-inflammatory activation.