Excision of DNA fragments with the piggyBac system in Chrysanthemum morifolium.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-06-25 DOI:10.5511/plantbiotechnology.23.0324a

Mitsuko Kishi-Kaboshi, Ayako Nishizawa-Yokoi, Ichiro Mitsuhara, Seiichi Toki, Katsutomo Sasaki

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Abstract

Chrysanthemum morifolium is one of the most popular ornamental plants in the world. However, as C. morifolium is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its trait systematically. Genome editing technology is one of the attractive methods for modifying traits systematically. For the commercial use of genetically modified C. morifolium, rigorous stabilization of its quality is essential. This trait stability can be achieved by avoiding further genome modification after suitable trait modification by genome editing. Since C. morifolium is a vegetatively propagated plant, an approach for removing genome editing tools is required. In this study, we attempted to use the piggyBac transposon system to remove specific DNA sequences from the C. morifolium genome. Using the luminescence as a visible marker, we demonstrated that inoculation of Agrobacterium harboring hyperactive piggyBac transposase removes inserted 2.6 kb DNA, which harbors piggyBac recognition sequences, from the modified Eluc sequence.

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应用piggyBac系统切除菊花DNA片段

菊花（Chrysanthemum morifolium）是世界上最受欢迎的观赏植物之一。然而，由于菊花是分段六倍体、自交不亲和、异源基因组庞大，因此很难对其性状进行系统改造。基因组编辑技术是有吸引力的系统改造性状的方法之一。要想将转基因大花蕙兰用于商业用途，就必须严格稳定其品质。通过基因组编辑技术对性状进行适当改造后，避免进一步的基因组改造，可以实现性状的稳定性。由于 C. morifolium 是一种无性繁殖植物，因此需要一种移除基因组编辑工具的方法。在这项研究中，我们尝试使用 piggyBac 转座子系统移除 C. morifolium 基因组中的特定 DNA 序列。利用发光作为可见标记，我们证明了接种携带超活性 piggyBac 转座酶的农杆菌可从修改后的 Eluc 序列中移除插入的 2.6 kb DNA，其中包含 piggyBac 识别序列。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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