Inhibition of esophageal cancer cell line (KYSE-30) proliferation using secondary metabolites of Ephedra endophyte bacteria

Abstract

Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition of esophageal cancer cell line (KYSE-30) proliferation were investigated. A total of 54 endophyte bacteria (out of 70) were obtained from the sterilized surfaces of two medicinal plants, i.e., Ephedra intermedia and Ephedra foliata. Bacterial strains were then cultured in Tryptic soy broth (TSB) medium and, after 72 h incubation, the produced secondary metabolites were extracted by chloroform. Anticancer effects of secondary metabolites from theses bacteria on esophageal cancer cell line KYSE-30 were evaluated after 24, 48 and 72 h by MTT method. MTT assay results showed that only strain A1 had a cytotoxic effect on KYSE-30 cells. The IC50 amounts of this strain against KYSE-30 cell lines were equaled (μg/ml) to 346.4, 192.8 and 121.3 after 24, 48, and 72 hours, respectively. The molecular identification of strain A1 revealed that Microbacterium maritypicum (99.8% Received 09.07.2020/ Revised 31.08.2020/ Accepted 19.11.2020/ Published 01.07.2021 :تفايرد ١٩ / ٤ ٠ / ٩ ١٣٩ / :حلاصا ٠ ١ / ٦ ٠ / ٩ ١٣٩ / پ :شريذ ٢٩ / ٨ ٠ / ٩ ١٣٩ / :راشتنا ١٠ / ٤ ٠ / ٤٠٠ ١ [ D O I: 1 0. 52 54 7/ nb r. 8. 2. 95 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .2 .1 .2 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 124 ]