Antifungal potential of biosurfactants produced by strains of Bacillus spp. (Bacillales: Bacillaceae) selected by molecular screening

IF 0.8 4区生物学 Q4 BIOLOGY Revista De Biologia Tropical Pub Date : 2022-09-09 DOI:10.15517/rev.biol.trop..v70i1.49716

Camila Olmedo, A. Koch, B. Sarmiento, Andrés Izquierdo

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引用次数: 1

Abstract

Introduction: Bacillus species are used as biological controllers for phytopathogenic fungi, and the mechanisms to produce controllers include biosynthesis of lipopeptide biosurfactants with antifungal activity. Objective: To evaluate the antifungal potential of the biosurfactants produced by Bacillus strains, selected by molecular screening, on Fusarium oxysporum. Methods: We selected four molecular markers, related to the biosynthesis of surfactin, fengicin, and lichenysin (srfA, spf, fenB, LichAA) in nine Bacillus strains. We used two mineral media with several culture conditions, for biosurfactant production, and a well diffusion test for antifungal potential. Results: Only the biosurfactant produced by UFAB25 inhibits the mycelial growth of F. oxysporum (44 % ± 13): this biosurfactant was positive for srfA, spf, and fenB genes involved in the synthesis of surfactin and fengicine. Antifungal activity depends on culture conditions and the strain. Conclusions: Genetic markers are useful to detect strains with antifungal potential, facilitating the selection of bio-controllers. The biosurfactant profile is influenced by the strain and by culture conditions.

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通过分子筛选筛选芽孢杆菌(杆菌科:芽孢杆菌科)产生的生物表面活性剂的抗真菌潜能

芽孢杆菌是植物病原真菌的生物控制者，其产生机制包括生物合成具有抗真菌活性的脂肽生物表面活性剂。目的:评价通过分子筛选筛选出的芽孢杆菌菌株生产的生物表面活性剂对尖孢镰刀菌的抑菌活性。方法:选取9株芽孢杆菌中与表面素、凤素、地衣素生物合成有关的4个分子标记(srfA、spf、fenB、LichAA)。我们使用了两种具有不同培养条件的矿物培养基，用于生产生物表面活性剂，并进行了抗真菌潜力的良好扩散试验。结果:只有UFAB25产生的生物表面活性剂对尖孢镰刀菌菌丝生长有抑制作用(44%±13%):该生物表面活性剂对参与表面素和枫碱合成的srfA、spf和fenB基因呈阳性反应。抗真菌活性取决于培养条件和菌株。结论:遗传标记可用于检测具有抗真菌潜力的菌株，便于生物防治剂的选择。生物表面活性剂的分布受菌株和培养条件的影响。

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来源期刊

Revista De Biologia Tropical 生物-生物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Revista de Biología Tropical / International Journal of Tropical Biology and Conservation is a mainstream scientific journal published since 1953 and covered by Web of Science; Science Citation Index; Current Contents; Google Scholar; Scopus, SciELO and nearly 50 additional indices. A double blind system guarantees you a fair evaluation, and our world class editorial and scientific boards provides a first decision in three working days. The journal is Full Open Access and is widely read where your article can have the highest real impact. Since its beginning in 1953, the Revista follows these principles: objective and independent evaluation of all manuscripts; transparency in all processes; ethical use of procedures, data, specimens and subjects; fair treatment of all parties; and absolute predominance of scientific rigor over any other aspect.