Variation in microbial biomass and enzymatic activities in metal contaminated soils during storage at low temperature (4°C)

IF 1.3 4区环境科学与生态学 Q3 ECOLOGY Chemistry and Ecology Pub Date : 2023-08-09 DOI:10.1080/02757540.2023.2253222

Alistar Moy, Kabwe Nkongolo

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Abstract

ABSTRACT Microbial response to soil storage at low temperature in limed and unlimed samples has not been investigated. For this study, soil samples were kept at 4°C for six weeks. Soil microbial biomass was determined using phospholipid fatty acid analysis (PLFA). Nine enzymes were targeted including β-glucosidase (BG), cellobiohydrolase (CBH), β-N-acetylglucosaminidase (NAGase), aryl sulfatase (AS), acid phosphatase (AP), alkaline phosphatase (AlP), glycine aminopeptidase (GAP), leucine aminopeptidase (LAP), and peroxidase (PER). PLFA results revealed a significant increase in total microbial biomass in limed area compared to unlimed soil samples. Microbial biomass decreased during the first two weeks of storage and remained unchanged thereafter for both limed and unlimed samples. Analysis of microbial activities revealed that most enzymes inconsistently decreased over time during storage at 4°C. Activities of BG, CBH, NAGase, AlP, AS, GAP, LAP were significantly higher in limed compared to the unlimed samples. Overall, the levels of activities of most enzymes in limed soils decreased significantly after the second week of storage and remained unchanged thereafter. The reduction of enzyme activity in the unlimed soil samples varied over time, with some enzymes such as LAP increasing on the sixth week. PER activity decreased after two weeks and increased thereafter.

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低温（4°C）储存期间，金属污染土壤中微生物生物量和酶活性的变化

微生物对土壤低温贮藏在石灰和未石灰样品中的反应尚未研究。在本研究中，土壤样品在4°C下保存6周。采用磷脂脂肪酸分析法(PLFA)测定土壤微生物生物量。9种酶包括β-葡萄糖苷酶(BG)、纤维素生物水解酶(CBH)、β- n -乙酰氨基葡萄糖苷酶(NAGase)、芳基硫酸酯酶(AS)、酸性磷酸酶(AP)、碱性磷酸酶(AlP)、甘氨酸氨基肽酶(GAP)、亮氨酸氨基肽酶(LAP)和过氧化物酶(PER)。PLFA结果显示，与未加石灰的土壤样品相比，石灰区微生物总生物量显著增加。微生物生物量在前两周的储存期间有所下降，此后在石灰和未石灰的样品中保持不变。微生物活动分析显示，在4°C的储存过程中，大多数酶随着时间的推移而不一致地减少。处理过的样品中BG、CBH、NAGase、AlP、AS、GAP、LAP活性显著高于未处理过的样品。总体而言，石灰土壤中大多数酶的活性水平在贮藏第二周后显著下降，此后保持不变。土壤样品中酶活性的降低随时间的变化而变化，其中一些酶如LAP在第6周有所增加。PER活性在两周后下降，随后升高。

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来源期刊

Chemistry and Ecology 环境科学-环境科学

CiteScore

4.20

自引率

16.00%

发文量

审稿时长

3.7 months

期刊介绍： Chemistry and Ecology publishes original articles, short notes and occasional reviews on the relationship between chemistry and ecological processes. This journal reflects how chemical form and state, as well as other basic properties, are critical in their influence on biological systems and that understanding of the routes and dynamics of the transfer of materials through atmospheric, terrestrial and aquatic systems, and the associated effects, calls for an integrated treatment. Chemistry and Ecology will help promote the ecological assessment of a changing chemical environment and in the development of a better understanding of ecological functions.