Morphology and gene expression of sex-determining region Y-box 9 and runt-related transcription factor 2 in centrifugally compressed cell collagen-combined constructs (C6)

IF 1.6 Q4 ENGINEERING, BIOMEDICAL Biosurface and Biotribology Pub Date : 2023-09-02 DOI:10.1049/bsb2.12062
Xu Ye, Masashi Yamazaki, Wataru Ando, Norimasa Nakamura, Hiromichi Fujie
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Abstract

The authors previously developed a scaffold-free tissue-engineered construct (TEC) from mesenchymal stem cells (MSCs). Although the TEC exhibited even cell distribution and was successfully applied for cartilage repair in animal models, it is unsuitable for relatively large-scale cartilage defects due to its small size. To solve the problem, the authors recently developed a novel biomaterial, a centrifugally compressed cell-collagen combined construct (C6) from a mixture of MSCs and atelocollagen, both of which are subjected to centrifugation. The results of the previous study indicated that C6 exhibited high cell viability (70 %) and sufficient cell distribution similar to that of the TEC. In the present study, the morphology and gene expression of C6 were investigated. Histological examination indicated that C6 is six times thicker (approximately 1 mm) than the TEC after a 7-day culture. The C6 remained unchanged in scale with increased cell density after a 21-day culture. Scanning electron microscopic observation indicated that C6 exhibited interconnected and porous microstructures, while the TEC had close-knit microstructures. Reverse transcriptase-polymerase chain reaction analysis indicated that the expression of sex-determining region Y-box 9 and runt-related transcription factor 2 was significantly higher in C6 than that in TEC.

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离心压缩细胞-胶原联合构建体中性别决定区Y‐box 9和runt相关转录因子2的形态学和基因表达(C6)
作者之前从间充质干细胞(MSC)中开发了一种无支架的组织工程构建体(TEC)。尽管TEC表现出均匀的细胞分布,并在动物模型中成功应用于软骨修复,但由于其体积小,不适用于相对较大的软骨缺损。为了解决这个问题,作者最近开发了一种新的生物材料,一种离心压缩的细胞-胶原组合构建体(C6),由MSCs和去端胶原的混合物制成,两者都经过离心。先前研究的结果表明,C6表现出高细胞活力(70%)和足够的细胞分布,与TEC相似。本研究对C6的形态和基因表达进行了研究。组织学检查表明,经过7天的培养,C6比TEC厚6倍(约1毫米)。C6在培养21天后保持不变,细胞密度增加。扫描电子显微镜观察表明,C6表现出互连和多孔的微观结构,而TEC具有紧密结合的微观结构。逆转录聚合酶链反应分析表明,C6中性别决定区Y‐box 9和runt相关转录因子2的表达显著高于TEC。
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来源期刊
Biosurface and Biotribology
Biosurface and Biotribology Engineering-Mechanical Engineering
CiteScore
1.70
自引率
0.00%
发文量
27
审稿时长
11 weeks
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