Biodistribution study of pantoprazole sodium in rodent tissues: A tool for pharmacokinetic study

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Journal of Reports in Pharmaceutical Sciences Pub Date : 2022-07-01 DOI:10.4103/jrptps.jrptps_71_22
Bhargavi V. Desai, P. Dedhiya, Ditixa M Desai, Ruchi Vyas, B. Vyas
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Abstract

Background: Pantoprazole sodium is one of the most widely used drugs for treating gastric acid–related disorders as well as is the most popular drug among various cancer therapy protocols for treating gastric disturbances pertained to the chemotherapy. The present study aims to validate high-performance liquid chromatographic (HPLC) method for the quantification of pantoprazole sodium in mice plasma and various tissue homogenates including kidney, heart, prostate, lung, pancreas, liver, and brain. Pantoprazole sodium estimation was done using 100 μL aliquot, which was injected into HPLC system, and the separation was achieved using Shimadzu C18 column at 40°C. Mobile phase composed of acetonitrile/dibasic phosphate buffer (40:60, v/v), pH = 7.4 was isocratically pumped at 1.0 mL min-1, and detection was performed at wavelength of 290 nm. Material and Methods: All the samples including tissues and plasma were collected after 4 h of oral administration of pantoprazole sodium to Swiss albino mice (10 mg/kg, p.o.). Results: Bioanalytical method was further validated according to the standard guidelines and portrays to be selective as well as linear (r2 ≥ 0.999) over the concentration range of 10–50 ng/injection. The intraday (% relative standard deviation [RSD] = 0.29%–1.21%) and inter-day precision (%RSD = 0.52%–2.88%) was found to be within the layout standards by International Council for Harmonization. Pantoprazole sodium extraction recovery was achieved between 64.15% and 78.17% demonstrating the suitability of the method. Conclusion: Bio-distribution study so carried out by bioanalytical technique can be used as an aiding tool for the quantification of pantoprazole sodium in all the studies involving the pharmacokinetic profiling of drug in various tissues of rodents.
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泮托拉唑钠在啮齿动物组织中的生物分布研究:药代动力学研究的工具
背景:泮托拉唑钠是治疗胃酸相关疾病最广泛使用的药物之一,也是各种癌症治疗方案中治疗化疗相关胃功能紊乱最常用的药物。本研究旨在验证高效液相色谱法(HPLC)定量小鼠血浆和肾、心、前列腺、肺、胰腺、肝、脑等组织匀浆中泮托拉唑钠的含量。泮托拉唑钠以100 μL的液相色谱法测定,进样于高效液相色谱系统,采用Shimadzu C18色谱柱分离,温度为40℃。流动相为乙腈/二碱磷酸缓冲液(40:60,v/v), pH = 7.4,等压泵入1.0 mL min-1,波长290 nm。材料与方法:采用泮托拉唑钠(10 mg/kg,口服)给药4 h后采集各组组织和血浆标本。结果:生物分析方法在10 ~ 50 ng/支的浓度范围内具有选择性和线性(r2≥0.999)。日内(%相对标准偏差[RSD] = 0.29% ~ 1.21%)和日间精度(%RSD = 0.52% ~ 2.88%)均在国际协调理事会的布局标准范围内。泮托拉唑钠提取回收率为64.15% ~ 78.17%,证明了该方法的适用性。结论:利用生物分析技术开展的泮托拉唑钠的生物分布研究,可作为泮托拉唑钠在啮齿类动物各组织药动学研究中定量的辅助工具。
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
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0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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