Comparative transcriptomes analysis of the ovary reveals potential ovarian development-related genes and pathways in Macrobrachium rosenbergii

Pub Date : 2022-12-26 DOI:10.1080/07924259.2022.2156822

Yan-Hao Yang, Rui Wang, Ming Li, Huizan Yang, Guang-Hua Huang, K. Ma, G. Qiu, Yong Lin

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Abstract

ABSTRACT The giant freshwater prawn (Macrobrachium rosenbergii), which is widely distributed in Southeast Asia and the western Pacific, is an important commercial prawn species. However, the molecular mechanism of ovarian development is unclear, and available data on the ovarian transcriptome of M. rosenbergii are limited. In this study, we constructed transcriptomes of female (ZW) and super female (WW) of M. rosenbergii ovaries to discover genes and pathways related to ovarian development. A total of 71,555 unigenes were obtained, with an average length of 1,530 bp and N50 of 3001 bp, and 15,792 (29.3%) of them were annotated. A total of 13,610 differentially expressed genes (DEGs) were detected. KEGG pathway analysis showed that multiple pathways related to ovarian development were significantly regulated, such as retinol metabolism, notch signalling pathway, cell cycle, wnt signalling pathway, FoxO signalling pathway, and mTOR signalling pathway. In addition, 12 putative ovarian development-related genes, including FZD9, DDR2, PSMC3IP, CREBBP, DMC1, WBP2NL, DHRS4, SPO11, BCDO2, SDR16C5, CPEB, RDH5, were identified. Our findings will provide a valuable archive for future functional analysis of genes related to ovarian development and future discoveries of the underlying molecular mechanisms of ovarian development.

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罗氏沼虾卵巢的比较转录组分析揭示了潜在的卵巢发育相关基因和途径

罗氏沼虾(Macrobrachium rosenbergii)是一种重要的商业对虾，广泛分布于东南亚和西太平洋。然而，卵巢发育的分子机制尚不清楚，关于罗氏乳杆菌卵巢转录组的可用数据有限。本研究构建了罗氏m.r onbergii卵巢雌性(ZW)和超级雌性(WW)的转录组，以发现与卵巢发育相关的基因和途径。共获得71,555个unigenes，平均长度为1,530 bp, N50为3001 bp，其中15,792个(29.3%)被注释。共检测到13610个差异表达基因(deg)。KEGG通路分析显示，与卵巢发育相关的多种通路，如视黄醇代谢、notch信号通路、细胞周期、wnt信号通路、FoxO信号通路、mTOR信号通路等均受到显著调控。此外，还鉴定出12个卵巢发育相关基因，包括FZD9、DDR2、PSMC3IP、CREBBP、DMC1、WBP2NL、DHRS4、SPO11、BCDO2、SDR16C5、CPEB、RDH5。我们的发现将为未来卵巢发育相关基因的功能分析和卵巢发育潜在分子机制的发现提供有价值的档案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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