{"title":"Aseptic Technique","authors":"Tomasz Bykowski, Jonathan F. Holt, Brian Stevenson","doi":"10.1002/cpet.31","DOIUrl":null,"url":null,"abstract":"<p>This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpet.31","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpet.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes. © 2019 by John Wiley & Sons, Inc.
无菌技术
本文介绍了可以降低培养物污染风险的常见实验室程序，统称为“无菌技术”。介绍了无菌工作的两种主要策略：使用本生燃烧器和生物安全柜。这两种方法都以通用方案的形式出现，适用于各种实验室任务，如移液和等分、生长培养基的制备、接种、传代和在培养皿上传播微生物。©2019 John Wiley&Sons，股份有限公司版权所有。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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