{"title":"Regulation of Plant Primary Metabolism – How Results From Novel Technologies Are Extending Our Understanding From Classical Targeted Approaches","authors":"A. Skirycz, C. Caldana, A. Fernie","doi":"10.1080/07352689.2022.2041948","DOIUrl":null,"url":null,"abstract":"Abstract The post-genomic era is characterized by a range of high throughput profiling methods capable of broadly characterizing gene expression levels, protein, and metabolite abundances. Application of these methods, enzyme profiling, and more recently, protein-metabolite interactions and flux analysis have alongside modeling approaches allowed us to refine our understanding of the regulation of metabolism even in the case of the canonical pathways of primary plant metabolism. Here we review recent insights obtained by using such methods in the context of our previous knowledge. In doing so, we hope to highlight the effectiveness of these methods and postulate that their application to less well-studied metabolic pathways will likely allow the elucidation of the hitherto unknown mechanism of metabolic regulation.","PeriodicalId":10854,"journal":{"name":"Critical Reviews in Plant Sciences","volume":"41 1","pages":"32 - 51"},"PeriodicalIF":6.0000,"publicationDate":"2022-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Critical Reviews in Plant Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/07352689.2022.2041948","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 2
Abstract
Abstract The post-genomic era is characterized by a range of high throughput profiling methods capable of broadly characterizing gene expression levels, protein, and metabolite abundances. Application of these methods, enzyme profiling, and more recently, protein-metabolite interactions and flux analysis have alongside modeling approaches allowed us to refine our understanding of the regulation of metabolism even in the case of the canonical pathways of primary plant metabolism. Here we review recent insights obtained by using such methods in the context of our previous knowledge. In doing so, we hope to highlight the effectiveness of these methods and postulate that their application to less well-studied metabolic pathways will likely allow the elucidation of the hitherto unknown mechanism of metabolic regulation.
期刊介绍:
Critical Reviews in Plant Sciences focuses on presenting in-depth and up-to-date reviews of timely and/or cutting-edge subjects in the broad discipline of plant science, ranging from molecular biology/biochemistry through the areas of cell biology, plant pathology and physiology, genetics, classical botany, and ecology, to practical agricultural applications. Articles in the journal provide an up-to-date literature base for researchers and students, pointing the way towards future research needs. The journal is also a significant source of credible, objective information to aid decision makers at all levels.