{"title":"Temperature and Time Optimization of pGEM-T Plasmid Transformation in Escherichia coli JM109","authors":"Fusvita Merdekawati","doi":"10.47307/gmc.2023.131.s4.8","DOIUrl":null,"url":null,"abstract":"Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmid transformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109.","PeriodicalId":35684,"journal":{"name":"Gaceta Medica de Caracas","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gaceta Medica de Caracas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47307/gmc.2023.131.s4.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
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Abstract
Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmid transformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109.
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Gaceta Médica de Caracas Órgano de la Academia Nacional de Medicina y del Congreso Venezolano de Ciencias Médicas Salud Fundada el 13 de marzo de 1893 Trimestral GMC
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