FIBRINOLYTIC POTENTIAL INCREASING DURING ACTIVATION OF BLOOD COAGULATION IN THE COURSE OF PREGNANCY WITH PLACENTAL DYSFUNCTION

Abstract

Aim. The study of molecular mechanisms of hemostasis balance is one of the most vivid tasks for clinical biochemistry. In present communication we aimed to underline the constant connection between blood coagulation and fibrinolysis. Methods. Blood coagulation activation was estimated by the soluble fibrin accumulation. For it determination we used the sandwich ELISA method. As the catch-antibody we used fibrin-specific mAb FnI-3C. As the tag-antibody we used another mAb (II-4d) that has an epitope in the NH 2 -terminal fragment of the γ-chain of the D-region of the fibrin(ogen) molecule. The rate of activation of fibrinolysis was estimated by measuring of Fibrinolytic Potential (FP). It was measured by turbidimetric method with recording the scattering of light by a fibrin clot at 405 nm on a microplate reader Multiscan (Finland). The clot was formed in the microplate wells in blood plasma activated by APTT reagent in the presence or without t-PA. Results. SF was found in blood plasma of 12 pregnant women with placenta dysfunction. Six of studied patients had SF less than 4 µg/ml that were assumed as the control meanings. We divided patients on two groups according to this parameter. It was shown that patients of the 1st group (SF ≤ 4) exhibited FP as 24 ou/s. In the same time patients of the 2nd group (SF ≥ 4) had much higher FP – 62 ou/s. The level of statistical significance was P = 0,05. Conclusions. Blood coagulation activation (estimated by SF measurement) was shown to be accompanied by fibrinolysis activity increasing (measured by FP evaluation) in pregnant women with placental dysfunctions. These findings can be evidence of constant balance between blood coagulation and fibrinolysis that stabilize hemostasis in pathological conditions for avoiding thrombosis or hemorrhages.