Study of triclabendazole effects on Fasciola hepatica’s life-supporting organs, spines and suckers responsible for stable position of the parasite in the host

Abstract

The purpose of the research is to study triclabendazole effects on the Fasciola’s life-supporting organs, spines and suckers which are responsible for stable position of the parasite in the host.Materials and methods. The study material was trematodes Fasciola hepatica (Linneus 1758, family Fasciolidae Railliet 1895), which were collected after the action of triclabendazole (fasinex) (chemically 5-chloro-6-(2,3-dichlorophenoxy)-2-methylthiobenzimidazole)on the 7th day after the drug administered at a single dose of 10 mg/kg for the Active Substance in the treatment of ovine fasciolosis. F. hepatica from untreated animals served as control. Mature F. hepatica collected after treatment with triclabendazole, and marita from the control groups were dehydrated in ascending alcohol series for 1–2 days after fixation; then passed through a mixture of chloroform and absolute alcohol (in a ratio of 1:1), and through pure chloroform in two portions for 10–15 minutes. The material was then soaked in a mushy mixture of chloroform and paraffin in a thermostat at 37 °C for 12–18 hours, and in paraffin in a thermostat at 56 °C for 30–45 minutes; and then embedded in paraffin with added wax. The resulting paraffin blocks were broken down into serial sections of 5–7 μm thick, then stained and examined under a light microscope.Results and discussion. Pathomicromorphological analysis of F. hepatica’s spines and suckers, organs that come into adhesive contact with the host organism revealed destructive changes in them after the action of triclabendazole. After the action of triclabendazole on fascioles, the spines look enlarged and swollen, and have a more rounded shape and some changes in color, absorbing eosin in greater concentration. The muscle fibers of the fascioles’ oral and abdominal suckers also look swollen after the action of triclabendazole. Although the musculature of the F. hepatica’s pharynx retained its structure, it has changes. It thickened sharply, which is clearly visible on the transverse and longitudinal sections of the helminths; neurosecretory cells are destroyed, and voids are observed in their place.