Metal-organic framework based on iron and terephthalic acid as a multiporous support for lipase Burkholderia lata LBBIO-BL02 and its potential for biocatalysis

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biocatalysis and Biotransformation Pub Date : 2022-05-02 DOI:10.1080/10242422.2022.2068371

A. M. Baron, Ricardo de Sousa Rodrigues, Luis Guilherme Giannina Sante, Jocácia Muriele de Miranda Kister, Valéria Marta Gomes do Nascimento, Alesandro Bail

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引用次数: 1

Abstract

Abstract Metal-organic frameworks (MOFs) are versatile materials because they have a large internal surface area and tuneable pores, making them suitable for enzyme immobilization. In this study, we prepared a typical microporous Fe-BDC MOF through a thermal treatment to produce additional meso and macropores interconnected to each other, capable of immobilizing the Burkholderia lata LBBIO-BL02 (BLL) lipase by entrapment and physical adsorption. The immobilization efficiency (E) was 90%, and the activity retention (R) was 400% (pNPP hydrolysis). The immobilized lipase (BLL@BDC) also showed excellent activity in the hydrolysis of vegetable oils in aqueous medium, achieving up to 3,200 U g−1 for olive oil, as well as high stability in organic solvents, especially for polar ones, such as iso-propanol (101.5 ± 2.6%), ethanol (103.0 ± 6.0%) and acetone (107.7 ± 8.3%). The results indicate that the multiporous Fe-BDC MOF is a promising support for lipase immobilization and further application in biocatalysis performed in organic media. Graphical Abstract

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基于铁和对苯二甲酸的金属有机骨架作为脂肪酶LBBIO-BL02的多孔载体及其生物催化潜力

摘要金属有机框架（MOFs）是一种多用途材料，因为它们具有大的内表面积和可调谐的孔，使其适合于酶固定化。在本研究中，我们通过热处理制备了一种典型的微孔Fe-BDC-MOF，以产生额外的相互连接的中孔和大孔，能够通过包埋和物理吸附固定拉塔伯克霍尔德菌LBBIO-BL02（BLL）脂肪酶。固定化效率（E）为90%，活性保留率（R）为400%（pNPP水解）。固定化脂肪酶(BLL@BDC)在水性介质中对植物油的水解也表现出优异的活性，达到3200 U g−1用于橄榄油，以及在有机溶剂中的高稳定性，特别是对于极性溶剂，如异丙醇（101.5 ± 2.6%）、乙醇（103.0 ± 6.0%）和丙酮（107.7 ± 8.3%）。结果表明，多孔Fe-BDC-MOF是一种很有前途的脂肪酶固定化载体，并在有机介质中的生物催化中进一步应用。图形摘要

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来源期刊

Biocatalysis and Biotransformation 生物-生化与分子生物学

CiteScore

4.40

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： Biocatalysis and Biotransformation publishes high quality research on the application of biological catalysts for the synthesis, interconversion or degradation of chemical species. Papers are published in the areas of: Mechanistic principles Kinetics and thermodynamics of biocatalytic processes Chemical or genetic modification of biocatalysts Developments in biocatalyst''s immobilization Activity and stability of biocatalysts in non-aqueous and multi-phasic environments, including the design of large scale biocatalytic processes Biomimetic systems Environmental applications of biocatalysis Metabolic engineering Types of articles published are; full-length original research articles, reviews, short communications on the application of biotransformations, and preliminary reports of novel catalytic activities.